蜂胶对氧自由基致鼠红细胞膜损伤的保护作用  

The Protective Action of Propolis Extract on Activity of Oxygen Free Radicals to Have Induced Erythrocyte Ghost Injury in Rats

作　　者：曹炜[1] 尉亚辉[1] 陈锦屏[2] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]陕西师范大学食品科学与工程系,西安710063

出　　处：《食品科学》2002年第5期118-120,共3页Food Science

基　　金：陕西省自然科学基金资助项目(2000SM16)

摘　　要：应用脂质过氧化法和荧光探针法研究了蜂胶对氧自由基致鼠红细胞脱膜氧化损伤的保护作用。结果表明,蜂胶能显著地抑制氧自由基引发的鼠红细胞膜脂质过氧化反应,脂质过氧化产物丙二醛(MDA)含量显著下降,呈现量效关系。用Fenton反应产生的羟基自由基攻击红细胞膜后,膜脂的流动性显著下降,当红细胞膜经40μg/ml的蜂胶预先处理后,膜脂的流动性显著提高,表明蜂胶对氧自由基引发的鼠红细胞氧化损伤有显著的保护作用。Both lipid peroxidation and fluorescence polarization were used to dertimne the protective action of propolis extract on the effect of rat erythrocyte ghost injury induced by oxygen-centred free radicals.The results showed that propolis could significantly inhibit lipid oxidation of rat erythrocyte ghost induced by action oxygen free radicals(peroxy radical and hydroxy radical)with dose-dependent effect.The erythrocyte ghost oxidative damage was induced by hydroxy free radical which could decrease membrance fluidity.But propolis(40μg/ml)could improve the membrance fluidity and inhibit the lipid peroxidation.These results showed that propolis could prevent lipid peroxidation in rat erythrocyte ghost damage induced by oxygen free dadicals.

关 键 词：蜂胶 氧自由基 鼠红细胞膜损伤 保护作用 抗氧化 保健食品资源 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] S896.6[轻工技术与工程—食品科学与工程]