原位杂交技术在检测染色体畸变中的研究  

THE STUDY ON CHROMOSOME ABNOMALITIES BY IN-SITU HYBRIDIZATION

作　　者：周俊[1] 翁立红[1] 顾文祥[1] 

机构地区：[1]浙江大学医学院组织胚胎学教研室,杭州310031

出　　处：《中国组织化学与细胞化学杂志》2000年第1期97-99,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：实验运用原位杂交技术检测肿瘤组织的染色体畸变。用 3、 7、 8、 9、 1 2号染色体着丝粒特异性 DNA探针 ,分别对外周静脉血的淋巴细胞、新鲜实体瘤组织、瘤组织石蜡切片进行染色体检测。二个染色体杂交点代表一对正常的染色体 ,三个染色体杂交点显示该细胞有染色体三体。整个实验过程简单、快速、准确、易重复。此方法和传统的细胞遗传学方法相结合 ,对探讨肿瘤的发生、发展有很大的意义。Chromosome abnormalities in tumor tissue were deetected by in situ hybridization. Using DNA probe specific for centromere region of chromosome 3,7,8,9 and 12, we have detected the lymphocyte of peripheral blood, the fresh tissue and the paraffin section of tumor tissue. We found that most cells had two hybridizaton signals, representing two normal chromosomes; some cells had three signals, representing an extra chromosome. The method was simple, rapid and stable. Under the ordinary light microscope, hybridization signals were clear, analysible. This technique in combination with traditional cytogenetics has great significance in discussing the development of tumor.

关 键 词：检测 染色体畸变 原位杂交 肿瘤组织 

分 类 号：R730.43[医药卫生—肿瘤] R596.1[医药卫生—临床医学]