WEB2基因参与酿酒酵母S期检查点调控  

A ROLE OF WEB2 GENE IN S PHASE CHECKPOINT REGULATION OF S.CEREVISIAE

作　　者：李新鸣[1] 孙黎光[2] 宣忠信 宋今丹[3] 

机构地区：[1]中国医科大学病原生物学教研室,沈阳110001 [2]中国中国医大生物化学与分子生物学教研室 [3]中国医科大学卫生部细胞生物学重点实验室

出　　处：《细胞生物学杂志》2002年第2期121-124,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金;39870384

摘　　要：WEB2基因编码产物为一种DNA解链酶。WEB2突变株经hydroxyurea阻断DNA合成后,经流式细胞仪检测DNA含量、纺锤体微管间接免疫 荧光染色及存活率测定,结果显示WEB2突变株表现S期检查点缺失。推测WEB2除了具有解链酶作用外,还参与酿酒酵母S期检查点调控机制,位于已建立的S期检查点信号传导通路模型上。本研究有助于加深对真核细胞检查点调控的了解,为研究肿瘤的发生机制提供线索。WEB2 gene encodes a DNA helicase. A WEB2 mutant was treated with Hydroxyurea. Facs analysis, spindle microtubule staining by indirect immunofluorescence and % survial indicate that the mutant is defect in S phase checkpoint. We propose that WEB2 has not only helicase activity, but a role in S phase checkpoint regulation. And WEB2 gene locates in S phase checkpoint signal transduction pathway in S. cerevisiae. The study should shed light on eukaryotic cell checkpoint regulation and the origin of cancer.

关 键 词：WEB2基因 酿酒酵母 S期检查点调控 

分 类 号：R730.2[医药卫生—肿瘤]