β-果糖基转移酶和β-呋喃果糖苷酶的分离纯化  

Purification of β-fructosyltransferase and β-Fructofuranosidase from Aspergillus niger SIPI-602

作　　者：杨正茂[1] 秦克亮[1] 赵玉秀[1] 杨光礼[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2002年第5期219-223,共5页Chinese Journal of Pharmaceuticals

摘　　要：用球磨机 Dyno- Mill破碎低聚果糖产生菌黑曲霉 (Aspergillus niger) SIPI- 6 0 2的菌丝体 ,其离心上清液经硫酸铵分级沉淀、Octyl- Sepharose CL - 4 B疏水层析、Q- Sepharose Fast Flow阴离子交换层析、Sephacryl S- 30 0凝胶过滤等步骤得到了在 SDS-聚丙烯酰胺凝胶电泳上均呈单带的两种酶 :β-果糖基转移酶与β-呋喃果糖苷酶。前者可将蔗糖转化成低聚果糖 ,而后者则催化蔗糖和低聚果糖的水解反应。用 SDS-聚丙烯酰胺凝胶电泳测得两者的分子量分别为 98.86 k D和 84 .92 k D。Purified β fructosyltransferase (1) and β fructofuranosidase (2) from a fructooligosaccharides producing strain, Aspergillus niger SIPI 602, showed only one band on SDS polyacr ylamide gel electrophoresis (SDS PAGE).The purification protocol consisted of ammonium sulfate precipitation and hydrophobic, anion exchange and gel filtration chromatographies. The molecular masses of 1 and 2 are 98.86 kD and 84.92 kD by SDS PAGE, respectively. 1 catalytically converts sucrose to fructooligosaccharides, while 2 hydrolyes sucrose and fructooligosaccharides to fructose and glucose.

关 键 词：低聚果糖 黑曲霉 β-果糖基转移酶 Β-呋喃果糖苷酶 分离纯化 

分 类 号：Q814.1[生物学—生物工程]