丹参酮对大鼠皮层神经细胞钙超载损伤的保护作用  

Effects of Tanshinone on calcium overloading injury in cultured primary cortex neurone

作　　者：何素冰 何丽娜[1] 杨军[1] 

机构地区：[1]安徽省医学科学研究所省生物医药重点实验室,安徽合肥230061

出　　处：《中成药》2002年第5期371-373,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :探讨丹参酮对大鼠神经细胞钙超载损伤模型的保护作用及其机制。方法 :采用组织培养法 ,以原代大鼠大脑皮层神经细胞为材料 ,制备咖啡因、KCI及NMDA诱导的钙超载损伤模型。结果 :形态学检查发现丹参酮对三种钙超载损伤模型中的大鼠神经细胞具有明显保护作用 ,结晶紫染色也提示丹参酮可显著提高损伤模型中神经细胞的存活数并降低细胞释放的LDH水平。结论Objective: To investigate the effects and mechanism of transhinone on calcium overloaded injuried models.Methods: Three injured models induced by caffeine, KCI, and NMDA, respectively, were used to assay the action of tanshinone in cultured primary cortex neurone of baby rats. Results: It was found that tanshinone possessed obvious protective effects on primary neurone in injured models by the way of morphological examination. Crystal violet staining and lactic dehydrogenase (LDH) measurement in supernate also indicated that tanshinone increased number of live neurone and reduced the extent of cell injury significantly.Conclusion: Tanshinone protected rat cortex cells from three kinds of calcium overloadied injured effectively in vitro.

关 键 词：丹参酮 神经细胞 钙超载 咖啡因 KCI NMDA 

分 类 号：R285[医药卫生—中药学]