芜菁花叶病毒温州分离物HC-Pro基因序列分析  

THE SEQUENCE ANALYSIS OF HC-PRO GENE OF TuMV-WZ ISOLATE

作　　者：施曼玲[1] 李桂新[2] 陶小荣[2] 

机构地区：[1]杭州师范学院生命科学学院,杭州310012 [2]浙江大学生物技术研究所,杭州310029

出　　处：《微生物学通报》2002年第1期26-30,共5页Microbiology China

基　　金：杭州师范学院科学研究基金资助项目(No.2001XC320);浙江省自然科学基金资助项目(No.301007)

摘　　要：　从浙江温州盘菜上获得芜菁花叶病毒温州分离物（TuMV-WZ）,病毒提纯后,经电镜观察,可发现大量长约700nm的线形粒子。利用免疫捕捉RT-PCR,通过特异性引物对TuMV-WZ的HC-Pro基因进行PCR扩增,经电泳可见1.5kb的特异条带。PCR扩增产物经过纯化后进行序列测定,序列长度为1449个核苷酸,编码482个氨基酸。其核苷酸序列与已报道的TuMV的其他分离物（Canada,UKl和Japanese）同源率分别为83.9％、96.5％和94.0％,氨基酸同源率分别为97.3％、98.8％和99.0％。A TuMV isolate (TuMV-WZ) was collected from Brasscia rapa L. in Wenzhou, Zhejiang Province. Observed under electron microscopy, many linear virions whose lengths were about 700nm could be found. By means of immunotrapping polymerase chain reation (PCR), TuMV-WZ HC-Pro gene was amplified by PCR with special primers. There was a 1.5kb special stripe in 0.8% agarose gel electrophoresis. After being purified, PCR Product was sequenced. The sequence result showed it was composed of 1449 nucleotides encoding a polypeptide of 482 amino acids. Nucleotide homogeneity of HC-Pro gene of TuMV-WZ isolate with those of other isolates (Canada, UK1 and Japanese) was 83.9%, 96.5% and 94.0%, respectively. Amino acid percentage similarity ofTuMV-WZ HC-Pro gene with other isolates was 97.3% , 98.8% and 99.0% , respectively.

关 键 词：温州分离物 序列分析 芜菁花叶病毒 HC-PRO基因 盘菜 

分 类 号：S432.41[农业科学—植物病理学]