血管活性肠肽对支气管上皮细胞趋化迁移的影响及机制  

Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells

作　　者：管茶香[1] 张长青[1] 秦晓群[1] 罗自强[1] 周伏文[1] 孙秀泓[1] 

机构地区：[1]中南大学湘雅医学院生理学教研室,长沙410078

出　　处：《生理学报》2002年第2期103-106,共4页Acta Physiologica Sinica

基　　金：TheprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 980 0 0 5 3 )

摘　　要：为探讨肺内神经肽在气道损伤修复中的作用 ,采用blind wellBoydenchamber测定原代培养的支气管上皮细胞 (bronchialepithelialcells,BEC)趋化性 ,观察血管活性肠肽 (vasoactiveintestinalpeptide ,VIP)对BEC趋化迁移的影响及其机制 ,并测定经热应激后BEC分泌VIP及表达VIP受体 (vasoactiveintestinalpeptidereceptor,VIPR)的变化。结果显示 :(1)以胰岛素作为趋化因子所建立的BEC趋化性测定方法稳定 ,重现性好 (r =0 970 3,P <0 0 1) ;(2 )VIP (0 0 0 1～ 1μmol/L)均显示剂量依赖性地增强BEC的趋化迁移 ,其效应可被钙调蛋白阻断剂及蛋白激酶C阻断剂有效地抑制 (P <0 0 1) ;(3) 4 2℃、30min热应激后BEC分泌VIP (P <0 0 1)及表达VIPR明显增加 (P <0 0 5 )。实验表明 :肺内神经肽VIP可增强BEC的趋化迁移 ,其细胞内信号转导途径与钙调蛋白及蛋白激酶C有关。To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind well Boyden chamber. Radioimmunoassay and radio ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible ( r =0 9703, P <0 01). (2) VIP (0 001～1 μmol/L) elicited chemotaxis of BECs which was substantial and concentration dependent. The effects of VIP were inhibited by W 7 and H 7 ( P <0 01). (3) Heat stress enhanced the secretion of VIP ( P <0 01) and upregulated the expression of VIPR on BECs ( P <0 05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.

关 键 词：血管活性肠肽 支气管上皮细胞 趋化性 血管活性肠肽受体 

分 类 号：R363[医药卫生—病理学]