肾虚衰老与线粒体DNA氧化损伤关系的研究  

Research on the relation between kidney deficiency senility and oxidzed injyry of mitochondrial DNA

作　　者：王学美[1] 富宏[1] 刘庚信[1] 

机构地区：[1]北京大学第一医院中西医结合研究所,北京西城区西安门大街1号100034

出　　处：《中医杂志》2002年第4期294-295,共2页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(39800199)

摘　　要：目的:观察肾虚衰老与外周血线粒体DNA缺失及线粒体呼吸链复合酶活力的相关性。方法:用聚合酶链反应(PCR)和酶动力学技术。结果:老年肾虚组线粒体DNA缺失较青年组和老年对照组明显增多、呼吸链复合酶Ⅰ、Ⅳ的活力较青年组和老年对照组明显降低。补肾方五子衍宗丸可明显减少线粒体DNA缺失、提高呼吸链复合酶Ⅰ、Ⅳ的活力。结论:提示线粒体DNA的氧化损伤可能是肾虚的重要原因之一。The relation between mitochondrial DNA (mtDNA) deletions, respiratory chain complex enzymes and kidney deficiency syndrome in the senility was studied by using polymerase chain reaction (PCR) and enzyme kinetic technique. Results indicated that the mtDNA deletion increased significantly and the activities of mitochondrial respiratory chain complexe enzyme I , IV in the senile kidney deficiency group decreased significantly as compared with the youth group and the senile control group. Wu Zi Yan Zong Pill, a prescription for tonifying the kidney, could reduce the mtDNA deletion and raise the activities of mitochondrial respiratory chain complexe enzyme I , IV in the senile kidney deficiency group. It is suggested that the oxidized injury of mtDNA can be regarded as one of the important reasons in kidney deficiency syndrome.

关 键 词：衰老 氧化损伤 肾虚 遗传学 DNA 线粒体 中医 

分 类 号：R228[医药卫生—中医基础理论]