银杏叶提取物中总黄酮测定方法的研究  

Study on the Determination Methed of the Flavonoids of Ginkgo Biloba Extract

作　　者：石红旗[1] 缪锦来[1] 韩丽[1] 张爱军[1] 吕培顶[1] 石书河[1] 

机构地区：[1]国家海洋局第一海洋研究所,青岛266061

出　　处：《食品科学》2002年第4期105-107,共3页Food Science

摘　　要：研究了银杏叶黄酮醇甙灌注色谱（　Ｐｅｒｆｕｓｉｏｎ　ｃｈｒｏｍａｔｏｇｒａｐｈｙ）测定方法。该方法采用外标定量法，使用ＹＷＧ－Ｃ１８反相柱，确定了较好的测定条件为：流动相甲醇－０．５％磷酸水溶液（６０／４０，ｖ／ｖ），流速１．５ｍｌ／ｍｉｎ，检测波长３６０ｎｍ。槲皮素浓度与峰面积呈线性关系的范围为１０～１８０μｇ／ｍｌ。平均回收率为９７．５％，ＲＳＤ为０．６７％。实验表明，黄酮醇甙水解经放置后，槲皮素含量下降，山奈酚含量上升，异鼠李素含量变化不明显，总黄酮醇甙含量呈下降趋势。The quantity of Ginkgo Flavonol Glycosides in GBE （Ginkgo Biloba Extract） was determined by FPLC techniques using external standard method. The column was packed with C18 (4.6μm×250mm). The mobile phase consisted of method-phosphate-water(60:5:35).The flow rate was 1.5ml/min and detection was affected at 360nm. It showed that the quantity of sample and the area of peak were in linear relationship from 2μg/ml to180μg/ml. The average recovery was 97.5%. The relative standard deviation was 1.60%. After the hydrolysis of the flavonoids , the content of quercentin decreased, but the content of kaempferol increased and isorhamnetin remained the same level.

关 键 词：银杏叶提取物 总黄酮 测定方法 灌注色谱测定 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]