多糖类药物对LAK细胞增殖功能的影响和抑瘤作用的实验研究  

Experimental studies on anti-tumor activity and effect of Ginseng polysaccharide on proliferative function of LAK cells

作　　者：谢遵江[1] 刘文庆[2] 方传龙[1] 张湛波[1] 刘颖[1] 贺业春[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150086 [2]齐齐哈尔医学院

出　　处：《中国肿瘤临床与康复》2002年第2期1-2,共2页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：黑龙江省教委科研基金资助项目 (编号 :980 0 0 12 )

摘　　要：目的　探讨多糖类药物对LAK细胞增殖功能的影响和抑瘤作用。方法　建立B16黑色素瘤小鼠模型 ,Ⅰ组瘤体周围皮下注射IL 2 ,Ⅱ组注射IL 2和人参多糖 ,Ⅲ组注射人参多糖 ,通过测量瘤重、瘤体积和抑瘤率观察其抑瘤作用 ;同时取小鼠脾细胞培养 ,1组加IL 2 ,2组加IL 2和人参多糖 ,3组加人参多糖 ,用台盼蓝染色 ,观察多糖类药物对LAK细胞增殖功能的影响。结果　人参多糖与IL 2联合体内应用抗肿瘤作用显著强于LAK细胞 (P <0 .0 5 ) ;人参多糖与IL 2联合体外培养表明 ,人参多糖能促进LAK细胞的增殖能力 (P <0 .0 5 )。结论　人参多糖与IL 2联合应用在体内抗肿瘤作用显著强于LAK细胞 。Objective To study on anti tumor activity and effect of Ginseng polysaccharide (Gp) on proliferative function of LAK cells.Methods The B 16 melanoma mouse model for research was established. The IL 2,IL 2 and Gp,Gp were injected into the mice subcutaneously in Ⅰ group,Ⅱ group,Ⅲ group respectively.After treatment,the changes of the tumor were detected.The spleen T cells mixed with IL 2,IL 2 and Gp,Gp were respectively cultured and the effects on proliferative function of LAK cells were observed.Results Gp combined with IL 2 in vivo had significantly anti tumor effect which was much higher than that of LAK cells(P<0.05);Gp and IL 2 cultured with T cells in vitro suggested that Gp could induce the proliferation of LAK cells.Conclusion Gp combined with IL 2 therapy is more effective than that of LAK cells in vivo,and Gp could promote the proliferation of LAK cells in vitro.

关 键 词：人参多糖 B16黑色素瘤 LAK细胞 抗肿瘤 抑瘤作用 实验研究 

分 类 号：R73-36[医药卫生—肿瘤]