用化学振荡(乳酸-丙酮-BrO_3^--Mn^(2+)-H_2SO_4体系)“探针”检测谷胱甘肽的抗氧化效应  

Determination of Activity of Antioxygenation of Glutathione by the "Probe" of the Lactic Acid(LA)-Acetone(Act)-BrO_3^--Mn^(2+)-H_2SO_4 Oscillating System

作　　者：杨树涛[1] 甘南琴[1] 林智信[1] 蔡汝秀[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《高等学校化学学报》2002年第6期1026-1029,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 75 0 18)资助

摘　　要：利用分析物脉冲微扰 (Analyte Pulse Perturbation,APP)技术研究了生物体内的一种抗氧化剂谷胱甘肽对乳酸 -丙酮 -Br O- 3-Mn2 + -H2 SO4 化学振荡体系振荡规律的影响 .采用这一振荡体系作为探针来检测GSH的抗氧化效应 ,考察了振荡体系中各反应物的浓度及各种因素的影响 ,它可作为定量分析的基础 ,线性范围为 1 .5 0× 1 0 - 7～ 2 .2 5× 1 0 - 5mol/L,检测限达 2 .2 0× 1 0 - 9mol/L,RSD为 1 .3 5 % (n=1 1 ) ,测定样品的速率为每小时 6～ 9个样品 。In our experiment, we applied APP technique to study of the perturbation of glutathione(GSH) which is an important antioxidant in vivo on the lactic acid(LA) Acetone(Act) BrO - 3 Mn 2+ H 2SO 4 oscillating reaction. And we determinate the activity of antioxygenation of GSH by the 'probe' of this oscillating system. Influence of experiment variables were investigated, and can be as a base of quantitative analysis. The linear range lies between 1.50×10 -7 -2.25×10 -5 mol/L, and the determination limit is 2.20×10 -9 mol/L. And the precision and throughput were also quite good(1.35% as RSD, and 6-9 samples/h, respctively). Some aspects of the potential mechanism of action GSH on the oscillating systems are discussed.

关 键 词：化学振荡 谷胱甘肽 乳酸 APP技术 抗氧化效应 抗氧化剂 人 衰老 丙酮 生理功能 

分 类 号：Q516[生物学—生物化学] R362[医药卫生—病理学]