大麦中蛋白质的快速测定法——碱浸紫外法  

THE QUICK DETERMINATION METHOD OF PROTEIN IN BARLEY --METHOD OF ALKALI EXTRACTING AND ULTRAVIOLET SPECTROPHOTOMETRY

作　　者：宋文军[1] 张燕[1] 张新棠 

机构地区：[1]天津轻工业学院食品工程系,天津300222

出　　处：《天津轻工业学院学报》2002年第2期22-24,共3页Journal of Tianjin University of Light Industry

摘　　要：大麦中蛋白质的经典分析法为凯氏定氮法 ,但操作繁杂费时 ,本文采用碱 (Na OH)浸取可溶性蛋白质 ,经紫外 (2 80 nm )比色 ,并以凯氏法测得结果为依据建立蛋白质含量与吸收值间回归方程 ,使检测方法简便、快速。Kjedahl determination is known as the classic analysis method for the determination of protein in barley.But this method is complex and takes much time.In this paper,the method of alkali extracting is used to extract soluble protein.After the determination with ultraviolet spectrophotometry,we obtain regression equation of protein content and absorption value according to the value of determination on kjedahl.This determination method is simple and quick in instructing industrial production.

关 键 词：大麦 蛋白质 快速测定法 碱浸紫外法 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]