8-Cl-cAMP对人食管癌细胞EGFR表达的影响  

Effets of 8-Cl-cAMP on the EGFR Expression in Human Esophageal Cancer Cells

作　　者：陈奎生[1] 王锦华[2] 雷冬梅[3] 张云汉[1] 

机构地区：[1]郑州大学医学院病理教研室河南省肿瘤病理重点实验室,河南郑州450052 [2]郑州市第三人民医院病理科,河南郑州450000 [3]郑州大学三附院病理科,河南郑州450052

出　　处：《河南大学学报（医学科学版）》2002年第1期4-5,共2页

基　　金：河南省自然科学基金资助项目 (994 0 2 130 0 )

摘　　要：目的 :探讨 8-Cl-cAMP对人食管癌细胞生长相关基因EGFR表达的影响。方法 :原代分组培养食管癌细胞后 ,采用完整细胞原位RNA斑点印迹技术对滴膜进行斑点印迹。结果 :经 8-Cl -cAMP处理的实验组EGFR的阳性信号较不加 8-Cl-cAMP处理的对照组EGFR的阳性信号弱 (P <0 .0 5 )。结论 :8-Cl-cAMP可下调与食管癌细胞生长相关的EGFR基因表达。Objective: To approach the effects of 8-Cl-cAMP on expression of EGFR in human exophageal cancer cells.Methods: After the human esophageal cancer cells were cultured at final concentration of 10 -5 mol/L 8-Cl-cAMP for 24h, both the experimental group cells and the control group cells were identified by using intact cell RNA dot blot techniques.Results: The gene expression of EGFR in the experimental group was weaker than that in the control group.Conclusion: The results shows that 8-Cl-cAMP can suppress the gene expression of EGFR in human esophageal cancer cells.

关 键 词：食管癌 8-Cl-cAMP EGFR 斑点印迹 基因表达 细胞信号传递系统 

分 类 号：R735.1[医药卫生—肿瘤]