番鸭细小病毒弱毒株VP2基因的序列分析  

Sequence analysis of VP2 gene of the attenuated strain of Muscovy duck parvovirus

作　　者：娄华[1] 白挨泉[1] 顾万军[1] 贺东升[2] 刘福安[2] 

机构地区：[1]佛山科技学院动物医学系,广东南海528231 [2]华南农业大学动物医学系,广东广州510642

出　　处：《中国兽医科技》2002年第6期6-10,共5页Chinese Journal of Veterinary Science and Technology

基　　金：广东省高等教育厅自然科学基金项目 (9742 )

摘　　要：将番鸭细小病毒强毒株MDPV Q经番鸭胚传代 ,获得致弱株MDPV 2 6,应用PCR技术扩增MDPV 2 6株的VP2基因片段 ,将扩增后的VP2基因重组到 pMD 18 T质粒载体上 ,并对插入片段进行序列测定 ,将测序结果及由该结果推导的氨基酸序列分别与MDPV Q株及国外已发表的MDPV相应序列进行同源性比较及分析。结果表明 ,MDPV 2 6与强毒株MDPVThe attenuated strain MDPV 26 had been derived from continued passages of virulent wild type (MDPV Q) in Muscovy duck embryos. The DNA encoding VP2 structural protein of MDPV 26 was amplified by PCR and recombined into T vector and sequenced . The comparison of VP2 gene sequence and amino sequence between MDPV 26 and MDPV Q, and MDPV registered in the gene bank had been carried out, respectively. It showed that both DNAs encoding VP2 structural protein of MDPV 26 and MDPV Q display 99.7% identity, and share 98.3% homology with that of the reported MDPV.

关 键 词：弱毒株 VP2基因 番鸭细小病毒 序列分析 PCR 同源性 

分 类 号：S852.659.2[农业科学—基础兽医学]