食物和饲料中生物素的测定方法  

DETERMINATION OF BIOTIN IN FOODS AND FEEDS

作　　者：石磊[1] 杨晓莉[1] 王国栋[1] 杨月欣[1] 

机构地区：[1]中国预防医学科学院

出　　处：《营养学报》2002年第2期181-184,共4页Acta Nutrimenta Sinica

基　　金：国家科技部资助项目

摘　　要：目的 :　建立食物和饲料中生物素的微生物测定法。方法 :　利用 Lactobacillusplantarum( ATCC80 1 4)的生长对生物素的依赖性 ,通过光密度法测定细菌生长和繁殖的强度来间接地测定食物或饲料样品中的生物素含量。结果 :　本方法的灵敏度为 0 .0 3ng,方法的批内相对标准偏差 ( RSD,Relative Standard Deviation)为 2 .2 %～ 3.8% ,批间 RSD为 2 .1 %～ 5 .3% ,回收率为 93.4%～ 1 0 4 .6% ,RSD为 3.1 %～ 4.1 %。结论 :　生物素的微生物测定法重现性好、准确度高 ,适用于食物和饲料中生物素的测定。Objective: To establish microbiological method for determining biotin in foods and feeds. Methods: Using biotin dependent microorganisms Lactobacillus plantarum(ATCC 8014), the biotin content was detected indirectly by the growth of cultrued bacteria spectrophotometrically.Results: The detection limit was 0.03 ng. The relative standard deviations(RSD) of within and between run assays were 2.2%-3.8%,2.1%-5.3% respectively. The recovery of added biotin was 93.4%-104.6%, and RSD was 3.1%-4.1%. Conclusion: This assay is sufficiently accurate and repoducible for determining biotin in foods and feeds.

关 键 词：食物 饲料 生物素 微生物法 光密度法 

分 类 号：O657.3[理学—分析化学]