脂质体介导C-myc基因反义寡核苷酸对人膀胱癌细胞系Biu-87的生物学影响  

Biological effects of liposome-mediated C-myc antisense oligodeoxynucleotides on human bladder cancer cell line Biu-87

作　　者：赵建华[1] 张绍增[2] 邵国兴[1] 董永超[2] 张钢[2] 张玉英[2] 常德辉[2] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710033 [2]兰州军区兰州总医院泌尿外科,甘肃兰州730050

出　　处：《西北国防医学杂志》2002年第4期285-287,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的 :探讨应用脂质体 (LR )介导C -myc基因反义寡核苷酸 (ASODN)对人膀胱癌细胞系Biu - 87的生物学影响。方法 :采用细胞计数、MTT比色法评价反义C -myc寡核苷酸对Biu - 87细胞体外增殖的影响 ,应用免疫组化 (SABC)、DAB显色检测C -myc反义寡核苷酸作用癌细胞后C -myc蛋白的表达情况。结果 :脂质体介导C -myc反义寡核苷酸组与对照组相比 ,Biu - 87细胞增殖活性受到明显抑制 (P <0 .0 5 ) ,12h后可抑制C -myc基因蛋白表达。结论 :脂质体介导C -myc反义寡核苷酸可抑制人膀胱癌细胞体外增殖活性。应用反义技术封闭C -myc癌基因表达 。Objective:To study the biological effects of liposome-mediated C-myc antisense oligodeoxynucleotides (ASODN) on human bladder cancer cell line Biu-87.Methods:The human bladder cancer cell line Biu-87 was treated with C-myc antisense oligodeoxynucleotides with lipofectin.The proliferation inhibition was studied with cell growth curves and MTT method. The expression of C-myc protein was detected by immunohistochemistry (SABC).Results:The cell proliferation was inhibited effectively by C-myc antisense oligodeoxynucleotides with lipofectin compared with the control groups (P<0.01). The protein expression of C-myc was inhibited completely at 12h.Conclusion:Antisense oligodeoxynucleotides conjugated with lipofectin might be a hopeful method to inhibit the proliferation of bladder cancer by inhibiting the expression of C-myc. Further investigations are needed to use this method in bladder cancer therapy.

关 键 词：膀胱癌 脂质体 C-MYC 反义寡核苷酸 

分 类 号：R737.14[医药卫生—肿瘤]