β-胡萝卜素对染镍肺泡巨噬细胞产生NO和NOS活性的影响  

Effects of β-carotene on Nitric Oxide and Nitric Oxide Synthase Production of Alveolar Macrophage Treated with Ni_2O_3

作　　者：龙继贤[1] 蔡亚滨[1] 邱水强[1] 刘晓庆[1] 李杰珍[1] 曾莉[1] 

机构地区：[1]同济大学医学院临床医学系,98级上海200072

出　　处：《同济大学学报（医学版）》2002年第3期215-217,共3页Journal of Tongji University(Medical Science)

摘　　要：目的　研究β 胡萝卜素拮抗三氧化二镍的细胞毒作用及机理。 方法　采用体外细胞培养法测定在 β 胡萝卜素 2 5 ,5 0 ,10 0 μmol/L) )作用下 ,体外染镍肺泡巨噬细胞 (alveolarmacrophage,AM)的存活率及活力。同时观察细胞内一氧化氮 (nitricoxide,NO)和活性氧的产生 ,以及一氧化氮合酶 (nitricoxidesynthase ,NOS)活力的变化。结果　在体外染镍肺泡巨噬细胞中加入不同浓度的β 胡萝卜后可提高该细胞的存活率和活力 ,可减少NO和活性氧的产生 ,降低NOS和活性 ,其中以 10 0 μmol/L的β 胡萝卜素最为明显。结论　镍可致细胞脂质过氧化 ,β 胡萝卜素具有拮抗镍对肺泡巨噬细胞的细胞毒作用 。Objective To study the effects of β-carotene on the injury of alveolar macrophages exposed to Ni 2O 3 in vitro.Methods The alveolar macrophages were cultured with exposure to Ni 2O 3 in vitro. Meanwhile,(25,50 and 100 μmol/L)β-carotene were added in the medium, respectively. The detection of nitric oxide(NO),active oxygen and activity of nitric oxide synthase(NOS) in alveolar macrophages were carried out. And the cell activity were determined after being exposed to Ni 2O 3 in vitro.Results The results revealed thatβ-carotene could decrease mortality and increase survival activity of alveolar macrophages. Active oxygen, NO and NOS activities were decreased at the same time . β-carotene at 100 μmol /L had the most dramatic inhibitory effect.Conclusion β-carotene can antagonize the lipid preoxidation of alveolar macrophage induced by Ni 2O 3 ,which probably is due to its antioxidant property.

关 键 词：染镍肺泡巨噬细胞 镍 Β-胡萝卜素 肺泡巨噬细胞 一氧化氮 一氧化氮合酶 活性氧 肺癌 

分 类 号：R734.2[医药卫生—肿瘤] R965[医药卫生—临床医学]