Cyclin E和p27^(kip1)在膀胱移行细胞癌中表达及与Ki-67表达的关系  

Research on the Expression of Cyclin E and P27^(kip1) in Bladder Transitional Cell Carcinoma and its Relation With the Expression of Ki-67

作　　者：杨登科[1] 陈书奎[1] 胡伟[1] 靳风烁[2] 李俊卿[3] 

机构地区：[1]解放军第159医院泌尿外科,河南驻马店463000 [2]第三军医大学附属大坪医院泌尿外科 [3]深圳市中心医院泌尿外科

出　　处：《中国医师杂志》2002年第7期690-692,共3页Journal of Chinese Physician

摘　　要：目的　探讨细胞周期调控基因蛋白cyclinE和p2 7kip1在膀胱移行细胞癌 (BTCC)中的表达及与Ki -6 7表达的关系。方法　采用免疫组化SP法 ,观察 6 5例BTCC中三种指标的表达情况 ,并用 12例正常黏膜作对比。结果　cyclinE在BTCC中阳性表达率随着病理分级 (P <0 0 5 )、临床分期和Ki- 6 7指数 (P <0 0 1)的升高而增高 ,有淋巴结转移组非常显著高于无淋巴结转移组 (P <0 0 1) ;而p2 7kip1在BTCC中的阳性表达率随着临床分期、病理分级和Ki- 6 7指数的增高而降低 (P <0 0 5 ) ,有淋巴结转移组显著低于无淋巴结转移组 (P <0 0 5 )。结论　cyclinE对BTCC的分化、增殖、浸润和转移可能起促进作用 ,而p2 7kip1则对BTCC的分化、增殖、浸润和转移可能起抑制作用。两者在细胞周期进展中的共同表达失调 ,可能是导致BTCC发生发展和向恶性表型转化的机制之一。联合检测cyclinE、p2 7Kip1和Ki- 6 7的表达对BTCC的生物学行为评价和判断预后具有重要意义。Objective To investigate the expression of cell cycle regulating protein cyclin E and p27 kip1 in bladder transitional cell carcinoma (BTCC) and to evaluate its relation with the expression of Ki-67.Methods With immunohistochemistry assay , the expressions of cyclin E , p27 kip1 and Ki-67 were examined in 65 cases of BTCC and 12 cases of normal bladder tissue as controls.Results There was a positive correlation between cyclin E expression and the pathological grade(P<0 05) , clinical stage , lymphoid node metastasis of BTCC and Ki-67 labeling index (P<0 01), and there was a negative correlation between p27 kip1 expression and the four indexes(P<0 05) . Conclusions Cyclin E may play a promoting role in the differentiation , proliferation, infiltration and metastasis of BTCC ;As contrast to cyclinE, p27 kip1 may play an inhibitive role in it . The disregulation of coexpression of the both cell cycle regulators may be one of the mechanisms of BTCC development and transformation to malignant phenotype. Examinations of cyclin E , p27 kip1 and K-67 expression in BTCC may have an important significance to judge its prognosis and biological behavior .

关 键 词：膀胱移行细胞癌 基因表达 CYCLIN E p^27kip1 Ki-67 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]