松针褐斑病菌毒素LA-Ⅰ和LA-Ⅱ致毒活性研究  

STUDIES ON THE PHYTOTOXIC ACTIVITIES OF LA-Ⅰ AND LA-Ⅱ PRODUCING BY BROWN SPOT NEEDLE BLIGHT FUNGUS (LECANOSTICTA ACICOLA)

作　　者：杨斌[1] 叶建仁[1] 包宏[1] 刘吉开[2] 董泽军[2] 

机构地区：[1]南京林业大学,南京210037 [2]中国科学院昆明植物研究所,昆明650204

出　　处：《林业科学》2002年第4期84-88,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目"松针褐斑病菌致病毒素的研究"(批准号 3 9870 62 7)

摘　　要：研究比较了松针褐斑病菌 (Lecanostictaacicola)两个致病毒素LA Ⅰ和LA Ⅱ的热稳定性、寄主专化性和最低有效浓度以及它们间的相互关系。LA Ⅰ和LA Ⅱ均具热稳定性 ,经沸水水浴加热 15min仍具活性。LA Ⅰ和LA Ⅱ不仅能伤害易感寄主植物湿地松、火炬松 ,对云南松、华山松、紫茎泽兰等也有伤害作用 ,说明LA Ⅰ和LA Ⅱ均为非寄主专化性毒素。LA Ⅰ和LA Ⅱ配成不同浓度溶液生测 ,显示LA Ⅰ对湿地松幼苗的最低有效浓度为 10 0 μg·mL- 1 ,LA Ⅱ的最低有效浓度为 5 0 0 μg·mL- 1 ,LA Ⅰ活性较LA Ⅱ的活性高 4～ 5倍。LA Ⅰ和LA Ⅱ混合液生测表明 。Brown spot needle blight of pine was an important disease in South China, which was caused by \%Lecanosticta acicola.\% The pathogen could produce two kinds of toxin, LA Ⅰ and LA Ⅱ. The toxin activities and basic properties of LA Ⅰ and LA Ⅱ were studied in the way of bioassay. The results indicated that LA Ⅰ and LA Ⅱ could not only injure the seedlings of host pine (\%Pinus elliottii,P.taeda\%) but also make the seedlings of other plants (\%P.yunnanensis,P.armandii\% and \%Eupatorium\% sp.) wilt. So both of the two toxins were non host specific toxins(NHST). LA Ⅰ and LA Ⅱ could keep their toxic activities for 15 minutes after boiling. The minimal effective concentrations of LA Ⅰ and LA Ⅱ were 100?μg·mL and 500?μg·mL respectively and their activities were not very strong. LA Ⅰ and LA Ⅱ could not affect their activities each other when they were mixed.

关 键 词：LA-I LA-Ⅱ 松针褐斑病菌 毒素 致毒活性 

分 类 号：S763.11[农业科学—森林保护学]