亚甲基四氢叶酸还原酶基因C677T多态性与胃癌易感性的关系  

Polymorphisms of methylenetetrahydrofolate reductase C677T and the risk of stomach cancer

作　　者：高长明[1] 吴建中[1] 丁建华[1] 刘燕婷[1] 臧宇[1] 李苏平[1] 苏平[1] 胡旭[2] 徐天亮[2] Takezaki Toshiro Tajima Kazuo 

机构地区：[1]江苏省肿瘤防治研究所流行病学研究室,南京210009 [2]淮安市卫生防疫站 [3]日本爱知县がんセンタ一研究所疫学,预防部

出　　处：《中华流行病学杂志》2002年第4期289-292,共4页Chinese Journal of Epidemiology

基　　金：日本文部省国际学术研究癌症特别研究经费资助项目 (0 80 42 0 15 )

摘　　要：目的　研究亚甲基四氢叶酸还原酶 (MTHFR)基因C6 77T多态性及其和烟酒嗜好相互作用与胃癌易感性的关系。方法　在上消化道癌高发区江苏省淮安市进行病例对照研究 (胃癌患者10 7例 ,对照人群 2 0 0名 ) ,调查研究对象的生活习惯 ,采用PCR RFLP技术检测研究对象的MTHFR基因型。结果　①胃癌组中MTHFR变异基因型拥有者的比例为 79.4 % ,显著高于对照组的 6 8.5 %(χ2 =4 .15 ,P =0 .0 4 16 )。MTHFR变异基因型拥有者发生胃癌的危险性显著升高 (OR =1.78,95 %CI :0 .99～ 3.2 2 ;性别和年龄调整OR =1.89,95 %CI:1.0 8～ 3.32 )。②在MTHFR变异基因型拥有者中 ,伴有吸烟习惯者发生胃癌的OR为 7.72 (95 %CI:2 .2 3～ 2 6 .79) ,伴有经常饮酒习惯者发生胃癌的OR为 3.0 8(95 %CI :1.30～ 7.2 3)。与不吸烟、不经常饮酒的野生型纯合子MTHFR基因型拥有者相比 ,伴有吸烟和经常饮酒习惯的MTHFR变异基因型拥有者发生胃癌的OR为 13.96 (95 %CI :2 .76～ 70 .4 6 )。结论　MTHFRC6 77T变异基因型与胃癌的易感性有关 ;Objective In order to study the relation between polymorphisms of methylenetetrahydrofolate reductase C677T (MTHFR) and susceptibility of stomach cancer (SC). Methods We conducted a case control study with 107 cases of SC and 200 population based controls in Huaian city of Jiangsu province, China. The epidemiological data were collected, and DNA of peripheral blood leukocytes was obtained from all of the subjects. MTHFR genotypes were detected by PCR RFLP method. Results (1) The frequency of MTHFR variant genotypes (C/T+T/T) among the cases ( 79.4% ) was significantly higher than the controls ( 68.5 %) ( P = 0.041 6 ); the crude OR for SC was 1.78 (95% CI : 0.99 3.22 ). After adjustment for sex and age,the OR for SC was 1.89 (95% CI : 1.08 3.32 ). (2)Subjects who had MTHFR variant genotypes and having smoking habit were at a significantly higher risk of developing SC ( OR = 7.72 , 95% CI : 2.23 26.79 ) compared with those who had wild type homozygotes (C/C) genotype and no smoking habit. Individuals who had variant genotypes and who had habit of frequent alcohol drinking were at an increased risk of developing SC ( OR = 3.08 , 95% CI : 1.30 7.23 ) compared with those with C/C genotype and low consumption of alcohol. As compared with subjects with C/C genotype and low consumption of alcohol and no smoking habit, individuals who had variant genotypes and who had habits of frequent alcohol drinking and smoking had 12.96 (95% CI : 2.76 70.46 ) folds risk developing SC. Conclusions These results in the present study suggested that the polymorphisms of MTHFR C677T was associated with risk of developing SC, and there was a coordinated effect between MTHFR genotypes and habits of smoking and alcohol drinking in the development of SC.

关 键 词：亚甲基四氢叶酸还原酶 胃癌 易感性 C677T基因 基因多态性 危险因素 基因变异 

分 类 号：R735.2[医药卫生—肿瘤]