测定浓缩菠萝汁中乳酸的毛细管气相色谱法  

Determination of Lactic Acid in Concentrated Pineapple Juice by Capillary Gas Chromatography

作　　者：黄惠玲[1] 张薇君[1] 林道颖 

机构地区：[1]海南出入境检验检疫局技术中心,海南海口570105

出　　处：《分析测试学报》2002年第4期85-87,共3页Journal of Instrumental Analysis

摘　　要：建立了毛细管气相色谱快速测定浓缩菠萝汁中乳酸的方法 ,样品经甲醇 -浓硫酸衍生化处理 ,四氯化碳萃取 ,采用氢火焰检测器 ,SUPELCOWAX_10石英毛细管柱 ,外标法定量 ,GC -MS确认;在0.01～1g/L的范围内乳酸含量与峰面积具有良好的线性关系 ,标准曲线的相关系数为0.999 ,检出限为0.008g/L,回收率为90%～96 % ,相对标准偏差为4.0 % ;方法具有快速、简便。A method for the determination of lactic acid in concentrated pineapple juice has been established by capillary gas chromatography. The lactic acid in sample is derivatized with methanol-sulfuric acid and extracted with carbon tetrachloride. A capillary column(SUPELCOWAX- 10) and flame ionization detector are employed. A external standard method is used for quantitative determination and GC-MS for qualitative determination. The linear range, correlation coefficient, detection limit, recoveries and relative standard deviation are 0.01～1 g/L, 0.999, 0.008 g/L, 90%～96% and 4.0%, respectively. The method proved to be simple, rapid, accurate and practical.

关 键 词：测定 浓缩菠萝汁 毛细管气相色谱法 乳酸 果汁 分析 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程] TS255.7[轻工技术与工程—食品科学与工程]