CD14在牙髓和牙龈组织中的免疫组化定位  

Immunohistochemical Localization of CD14 in human dental pulp and gingival tissues

作　　者：王胜朝[1] 荫俊[2] 史俊南[1] 范继红[1] 宋伟[2] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《牙体牙髓牙周病学杂志》2002年第7期352-354,I001,共4页Chinese Journal of Conservative Dentistry

基　　金：军事医学科学院微生物流行病研究所承担国家自然科学基金资助项目 (3 9870 3 16)

摘　　要：目的 :检测CD14在牙髓和牙龈组织的分布 ,探讨CD14在牙髓和牙龈炎症过程中的可能作用。方法 :组织切片技术制备人牙髓和牙龈组织标本 ,用免疫组织化学方法检测CD14在健康和炎症牙髓、牙龈组织的分布。结果 :健康牙髓没有发现CD14免疫阳性细胞 ,炎性牙髓组织病灶处炎细胞呈阳性反应 ;健康牙龈上皮棘层细胞有微弱的阳性反应 ,炎性牙龈上皮棘细胞层强阳性。结论 :CD14参与了牙髓和牙龈组织的炎症反应过程 ;特别是牙龈上皮棘细胞层CD14免疫阳性 。AIM: To investigate the distribution of CD14 in dental pulp and gingival tissues and possible roles of CD14 in inflammation of these tissues. METHODS: Dental pulp and gingival tissues were obtained from patients and healthy volunteers. Tissue slices were routinely prepared. ABC assay was performed to detect CD14 in healthy and inflammatory conditions. RESULTS: CD14 was not detected in healthy pulp tissues. Only macrophages were positively stained in inflammatory pulps. Positive-stained cells were found in both normal and inflammatory gingival epithelium, but in the latter that was much stronger. CONCLUSION: CD14 may play an important role in the process of dental pulpitis and gingivitis.

关 键 词：牙髓 牙龈 牙髓炎 牙龈炎 免疫组织化学 白细胞分化抗原-14 

分 类 号：R781.42[医药卫生—口腔医学] R781.31[医药卫生—临床医学]