多粘类芽胞杆菌利用生物柴油副产物粗甘油生产乙偶姻  

Production of acetoin by Paenibacillus polymyxa from biodiesel derived glycerol

作　　者：杨晗[1] 纪晓俊[1] 聂志奎[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009

出　　处：《生物加工过程》2014年第4期11-15,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21006049);国家高技术研究发展计划(863计划)(2011AA02A207);江苏高校优势学科建设工程

摘　　要：研究多粘类芽胞杆菌利用廉价底物生物柴油副产物粗甘油批次发酵生产乙偶姻,考察不同pH条件、不同转速下乙偶姻、2,3-丁二醇和乙酸3种主要产物的产量,根据发酵结果设计一种三阶段溶氧调节的方法,结果表明:经76h批次发酵,乙偶姻产量为14.62 g/L,副产物2,3-丁二醇和乙酸分别为1.24和2.93 g/L;副产物量低,且易于分离,可以有效减少后期分离提取产物的成本。Biodiesel derived glycerol was used as substrate in batch fermentations by Paenibacillus polymyxa for producing acetoin.Major products, such as acetoin, 2, 3-butanediol and acetate were investigated in different initial pH,constant pH, and agitation speed conditions.According to the results of batch experiments,a three stage fermentation was designed,after 76 h batch fermentation,acetoin yield reached to 14.62 g/L.The yield of 2, 3-butanediol and acetic acid were 1.24 g/L, 2.93 g/L, respectively.Small amount of by-products could be easily separated to reduce costs of extraction.

关 键 词：甘油 多粘类芽胞杆菌 生物柴油 乙偶姻 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ655