ChIP-seq比较分析人体心脏和脾脏的H3K9三甲基化差异  

Analysis of difference in histone H3K9 trimethylations in normal human heart and spleen by ChIP-seq

作　　者：薛雯[1] 曹翠辉[1,2] 眭维国[1] 车文体[1] 陈洁晶[1] 郭丽[1] 戴勇 

机构地区：[1]中国人民解放军第181医院 肾脏科 广西代谢性疾病研究重点实验室,广西桂林541002 [2]广西师范大学生命科学学院,广西桂林541004 [3]深圳市人民医院 [4]暨南大学 第二临床医学院临床医学研究中心,广东深圳518020

出　　处：《基础医学与临床》2014年第7期939-944,共6页Basic and Clinical Medicine

基　　金：广西自然科学基金(2012GXNSFDA053017);广西科技基础设施建设项目(11-031-33);广西重点实验室建设项目(12-071-32)

摘　　要：目的分析人体器官心脏和脾脏的H3K9me3的全基因图谱,以期发现H3K9三甲基化的修饰与组织特异性的表达、功能和发育具有相关性。方法通过染色质免疫共沉淀的方法获取各标本DNA,通过q PCR验证ChIP结果,然后构建ChIP Sequencing文库,与目的基因组序列比对,获取比对Reads,接着进行全基因组的Peak分析,GO功能富集分析peak相关基因的生物学功能。结果心脏和脾脏间有169个基因有显著地H3K9me3差异,其中心脏中H3K9me3的基因中有64个特殊基因,脾脏中H3K9me3的基因中有87个特殊基因。从这些基因中,挑选出8个表现H3K9me3明显的基因,然后再从这8个基因中挑选出两个心脏和脾脏间表现H3K9me3差异最明显的基因,分别是PTPN3和RBMS。结论 H3K9me3差异可作为一个潜在的生物标志物或是表观遗传疾病的治疗靶点。Objective The modifications are closely-associated with tissue-specific expression,function and development by generating the genome-wide maps of H3K9me3 of human heart and spleen.Methods The DNA of samples was extracted by chromatin immunoprecipitation.The ChIP results were validated by qPCR.Then building the ChIP sequencing library,contrasting with the target genome sequence to obtain compared reads,and peak analysis of whole genome,GO enrichment analysis of the biological functions of the related genes of peak.Results 169 genes displayed significant H3K9me3 differences between heart and spleen.Among these genes,64 genes were the special genes in heart-H3K9me3 ; 87 genes were special genes in spleen-H3K9me3.From these genes,we selected eight genes of H3 K9 which were of significantly expression.Then selecting two genes of H3 K9 which were the most significant difference expression from the eight genes.They are PTPN3 and RBMS.Conclusions H3K9me3 may be a potential biomarker or promising target for epigenetic-based disease treatment.

关 键 词：H3K9me3 CHIP-SEQ 表观遗传学 

分 类 号：R394[医药卫生—医学遗传学] Q78[医药卫生—基础医学]