高效液相色谱法检测乳制品中的叶黄素  

Determination of Lutein in Dairy Products by HPLC

作　　者：赵贞 高敏 万鹏 吕海燕 陈静 李翠枝 

机构地区：[1]内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010010

出　　处：《乳业科学与技术》2014年第3期16-18,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金："十二五"国家科技支撑计划项目(2012BAK17B16)

摘　　要：建立了乳制品中的叶黄素液相色谱分析方法.样品经丙酮提取后,以甲醇-甲基叔丁基醚为流动相,反相色谱分离,紫外检测器445 nm进行检测,外标法定量.该方法具有良好的准确度和精密度,回收率为86.9％～101.2％,相对标准偏差为2.56％～4.05％,定量检出限0.02 mg/kg,线性范围0.02～4 mg/kg.A method for determining lutein in dairy products by HPLC is described.Samples were extracted with acetone and separated with a mobile phase made up of methanol and methyltert-butyl ether.The analysis was performed by UV detection at 445 nm,and the external standard method was used for quantification.This method has good accuracy and precision.The spiked recoveries in milk and milk powder ranged from 86.9％ to 101.2％ with a relative standard deviation of 2.56％ to 4.05％.The detection limit was 0.02 mg/kg,and a linear range of 0.02 to 4 mg/kg was obtained.

关 键 词：商效液相色谱法 叶黄素 乳制品 

分 类 号：O657.72[理学—分析化学] TS252.7[理学—化学]