超高效液相色谱-串联质谱法测定猪肉、猪肝及猪尿中的阿托品残留  

Determination of Atropine in Porcine Muscle,Liver and Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：胡兴娟[1,2] 吴宁鹏[2] 孟蕾[2] 彭丽[2] 李慧素[2] 班付国[2] 

机构地区：[1]郑州大学,郑州450001 [2]河南省兽药监察所,郑州450008

出　　处：《中国兽药杂志》2014年第7期46-49,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了猪肉、猪肝和猪尿中阿托品残留的超高效液相色谱-串联质谱（ UP LC-MS/MS ）的检测方法。样品经乙腈-0．1％甲酸水（ V/V，90∶10）溶液提取，Agela Cleanert？ PEP-2固相萃取柱净化后，采用UPLC-MS/MS测定，外标法定量。结果表明，阿托品在0．2～10 ng/mL范围内线性关系良好，相关系数大于0．99。在0．5～2．5μg/kg 添加浓度范围内阿托品的批间平均回收率78．3％～98．2％，批内、批间相对标准偏差均小于15％，检测限为0．2μg/kg，定量限为0．5μg/kg。该方法简便、灵敏度高、重现性好，适用于猪肉、猪肝及猪尿中阿托品含量的测定。A method based on ultra performance liquid chromatography coupled with tandem mass spectrometry （ UPLC-MS/MS） was developed for determination of atropine in porcine muscle,liver and urine. The analyte was extracted with acetonitrile-0.1%formic acid in water （ V/V,90 ∶ 10） ,and was cleaned up by the Agela Cleanert？ PEP-2cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring （ MRM） using external standard. The results indicated that the correlations were greater than 0.99 at the range of 0.2~10 ng/mL. The average recoveries of the drug ranged from 78.3%~98.2%at spiked levels of 0.5~2.5μg/kg with RSD less than 15%. The detection limit and quantification limit was 0.2μg/kg and 0.5μg/kg, respectively. The method was convenient, sensitive and reproducible for monitoring atropine residues in porcine muscle,liver and urine.

关 键 词：猪肉 猪肝 猪尿 阿托品 残留 超高效液相色谱-串联质谱法 

分 类 号：S859.84[农业科学—临床兽医学]