促红细胞生成素对大鼠肢体缺血再灌注后肾血流的影响  

The Influence of Erythropoietin in Renal Blood Flow after Limb Ischemia Reperfusion in Rats

作　　者：刘彩树 李为朋[1] 闫合燕 张亚萍[1] 蔡森[1] 门秀丽[1] 孔小燕[1] 李宏杰[1] 

机构地区：[1]河北联合大学基础医学院,唐山063000

出　　处：《天津医药》2014年第7期661-663,共3页Tianjin Medical Journal

基　　金：河北联合大学大学生创新性实验计划资助项目(X2013037);河北联合大学培育基金项目(LDPYS05;GP201301)

摘　　要：目的探讨促红细胞生成素(EPO)对大鼠肢体缺血再灌注(LIR)后肾血流的影响及可能作用机制。方法30只雄性SD大鼠随机分为Control组、LIR组和EPO+LIR组,每组10只;检测各组动物肾血流量、血浆肌酐(Cr)、尿素氮(BUN)含量、肾组织湿干比(W/D)、NO、一氧化氮能合成酶(NOS)及内皮素-1(ET-1)含量,采用免疫组织化学法检测肾组织细胞间黏附分子(ICAM-1)和血管细胞黏附分子(VCAM-1)的表达,光镜下观察肾组织形态学改变。结果 LIR组较Control组肾血流量减少,血浆Cr、BUN、肾组织W/D、NO、ET-1、NOS活性、ICAM-1和VCAM-1的表达增高(P<0.05),镜下可见肾组织间隙增宽和炎细胞浸润等病理改变。EPO+LIR组较LIR组肾血流量增加,血浆Cr、BUN、肾组织W/D、NO、ET-1和NOS活性、ICAM-1和VCAM-1的表达均降低(P<0.05),肾组织病理学变化有所减轻。结论 EPO可改善LIR后大鼠的肾功能,增加肾血流量,其机制可能与减轻肾水肿、调节肾血管舒缩功能、减轻炎症反应有关。Objective To investigate the influence and mechanism of erythropoietin （EPO） in renal blood flow after limb ischemia reperfusion （LIR）. Methods Thirty male SD rats were randomly divided into control group, LIR group and EPO＋LIR group with ten in each group. The values of renal blood flow, plasma creatinine （Cr）, urea nitrogen （BUN） content in plasma, kidney tissue wet to dry ratio （W/D）, nitric oxide （NO）, nitric oxide synthase （NOS） and endothelin-1 （ET-1） in re-nal tissue were detected in three groups. The immunohistochemistry assay was used to detect the expression of intercellular adhesion molecule （ICAM-1） and vascular cell adhesion molecule （VCAM-1） in renal tissue. The morphological changes of renal tissue were observed with light microscope. Results The renal blood flow was significantly decreased, while the val-ues of Cr, BUN, W/D, NO, ET-1, NOS, expressions of ICAM-1 and VCAM-1 was significantly increased in LIR group than those of control group （P〈0.05）. Broaden interstitial and infiltration of inflammatory cells were observed in the renal tissue under light microscope. In the EPO＋LIR group, the renal blood flow increased, the values of Cr, BUN, W/D, NO, ET-1 and NOS, expressions of ICAM-1 and VCAM-1 decreased significantly compared with those of LIR group （P〈0.05）. The patho-logical changes were alleviated in EPO＋LIR group. Conclusion EPO can improve renal function, increase renal blood flow in rats after LIR. The mechanism may be related to the decreased edema, changed renal vasomotor function and decreased in-flammation.

关 键 词：再灌注损伤 四肢 肾血浆流量 有效 红细胞生成素 大鼠 SPRAGUE-DAWLEY 疾病模型 动物 

分 类 号：R363.2[医药卫生—病理学] R977.1[医药卫生—基础医学]