聚乙二醇-硫酸铵双水相体系分离纯化黄花蒿黄酮的研究  

Separation and purification of flavonoids from Artemisia annua L.by polyethylene glycol-ammonium sulfate aqueous two-phase system

作　　者：欧阳文[1,2] 王家坚 熊利芝[1,2] 

机构地区：[1]吉首大学/植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《食品工业科技》2014年第15期196-199,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(51262008;51202087;51364009);湖南省自然科学基金(12JJ2005;14JJ4048);湖南省科技厅工业支撑计划重点项目(2012GK2017);湖南省教育厅重点项目(12A109);湖南省高校创新平台开放基金项目(13K094);大学生研究性学习与创新性实验计划(JSU-CX-2012-39);返校博士科研资助经费研究项目(jsdxxcfxbskyxm201111);植物资源保护与利用湖南省高校重点实验室开放基金(JSK201108);湖南省重点学科建设项目

摘　　要：采用聚乙二醇-硫酸铵双水相体系分离纯化黄花蒿黄酮。通过单因素实验考察聚乙二醇平均分子量、(NH4)2SO4质量分数、聚乙二醇质量分数、pH和体系温度等对分配系数、萃取率的影响,并通过正交实验对萃取工艺条件进行进一步的优化,获得最佳工艺条为:双水相体系组成30%PEG600-25%(NH4)2SO4,体系温度45℃,体系pH为3。在此条件下,黄花蒿黄酮主要分布在上相,分配系数为21.86,萃取率达到96.33%。An aqueous two-phase system of PEG-（ NH4）2SO4 was used to extract flavonoids from the crude ethanol extract of Artemisia annua L..The effects of average molecular weight of PEG, mass percent of（ NH4 ）2SO4, mass percent of PEG,pH and temperature on partition coefficient and extraction rate of flavonoids were explored by single factor experiments, then the extraction condition of flavonoids was further optimized by orthogonal method.Then the optimum conditions were obtained as follows： An aqueous two-phase system of 30% PEG600 and ：25% （NH4 ）2SO4, pH of 3 and temperature of 45℃.Under the optimal extraction conditions, flavonoids from Arternisia annua hwas mainly distributed in the upper phase with a partition coefficient of 21.86 and the extraction rate of 96.33%.

关 键 词：双水相体系 分离纯化 黄花蒿 黄酮 

分 类 号：TS201.1[轻工技术与工程—食品科学]