番石榴叶具有α-葡萄糖苷酶抑制活性的倍半萜杂二醛分离与鉴定  

Sesquiterpenoids with α-Glucosidase Inhibitory Activities from the Leaves of Psidium guajava Linn.

作　　者：欧阳文[1] 朱晓艾[2] 刘润南[1] 刘晓娟[2] 粟龙杰 谢磊[1] 曹庸[2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]华南农业大学食品学院,广东广州510642

出　　处：《湖南中医药大学学报》2014年第8期17-20,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学青年基金项目(31201321);湖南省教育厅项目(13B084);广州市科技计划项目(2013J4100075);湖南中医药大学本科教学质量工程项目(TD-007)

摘　　要：目的分离纯化番石榴叶乙酸乙酯萃取物,并对其抑制α-葡萄糖苷酶活性作用进行研究。方法采用硅胶、凝胶柱层析分离纯化,波谱法进行结构鉴定,比色法进行活性测定。结果分离得到三种倍半杂萜二醛,分别鉴定为psidials A,psiguadial A和guajadial;活性测试结果显示guajadial具有抑制α-葡萄糖苷酶活性,且显著优于阿卡波糖。结论首次报导guajadial为番石榴叶具有抑制α-葡萄糖苷酶活性的成分。Objective To study the α-glucosidase inhibitory active compounds from the ethyl acetate extract of guava leaves. Methods The compounds were separated from the ethyl acetate extract by repeating silica gel and Sephadex LH-20 column chromatography. The 1H-NMR, 13C -NMR techniques were used to identify the chemical structures. The α-glucosidase inhibitory activities were tested by colorimetry. Results Three special meroterpenoids were isolated and identified as psidials A, psiguadial A and guajadial. Guajadial showed α-glucosidase inhibitory activities significantly and its activity was better than that of Acarbose. Conclusions Guajadial as one of the α-glucosidase inhibitory active compound from Guava leaves was reported for the first time.

关 键 词：番石榴叶 倍半萜杂二醛 分离鉴定 Α-葡萄糖苷酶 

分 类 号：R284.1[医药卫生—中药学]