茶多酚对酒精性肝损伤过氧化的影响  

Effect of tea polyphenols intake on ethanol-induced liver injury

作　　者：唐袁婷[1] 管小琴[2] 江咏梅[1] 岳新爱[1] 陈平[1] 彭英[1] 玉洁[1] 

机构地区：[1]四川大学华西第二医院检验科,成都610041 [2]重庆医科大学附属第一医院病理科,400016

出　　处：《重庆医学》2014年第21期2736-2738,共3页Chongqing medicine

摘　　要：目的建立酒精性肝损伤的细胞模型,研究茶多酚(TP)对酒精性肝损伤的调节作用。方法通过细胞培养,采用显微镜观察细胞形态,检测细胞培养液丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(GGT)和细胞内活性氧(ROS)变化。结果乙醇使L02肝细胞脂肪变性。与乙醇组比较,TP+乙醇组脂肪变性轻,其培养液中ALT、AST、GGT水平降低,细胞内ROS减少。结论体外实验中茶多酚能减轻细胞脂肪变性程度,改善酶学指标,减少ROS的产生,来避免肝脏损害。Objective To establish the cell model of ethanol-induced liver injury and explore the protective effects of tea poly-phenols （TP）on ethanol-induced liver injury .Methods Cell morphology were observed by microscope ,and then alanine aminotrans-ferase （ALT） ,nmda transaminase （AST） ,gamma GGTP ,GGT and ROS changes were detected .Results Alcohol maked L02 hepa-tocyte fatty degeneration .Compared with ethanol group ,steatosis in TP ＋ ethanol group was lighter ,its ALT ,AST ,GGT content and intracellular ROS reduced .Conclusion TP can decrease cell fatty change degree in vitro experiments ,improue the enzymology indexes ,reduce the generation of reactive oxygen species to avoid liver damage .

关 键 词：茶多酚 酒精性肝损伤 过氧化 

分 类 号：R575.5[医药卫生—消化系统]