荧光免疫层析法快速测定牛奶中头孢氨苄残留量  

Rapid detection of cefalexin residues in milk by fluorescent immunochromatography assay

作　　者：胡佳丽[1,2] 于东升[3] 刘小雷[1] 云彩麟[1] 李莉[2] 刘彩虹[2] 张帆[2] 陈艳[2] 邹明强[2] 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010059 [2]中国检验检疫科学研究院,北京100123 [3]内蒙古精神卫生中心,内蒙古呼和浩特010010

出　　处：《中国食品卫生杂志》2014年第4期362-366,共5页Chinese Journal of Food Hygiene

基　　金："十二五"国家科技支撑计划(2012BAK26B04);质检公益性行业科研专项(201010022)

摘　　要：目的采用荧光免疫层析法结合现场检测仪建立牛奶中头孢氨苄残留的快速定量检测方法。方法利用反向微乳技术合成稀土铕荧光纳米颗粒，与头孢氨苄单克隆抗体结合制备荧光检测探针，以头孢氨苄卵清蛋白全抗原和羊抗小鼠抗体分别作为检测线和质控线制备免疫层析试纸务，结合现场检测仪建立牛奶中头孢氨苄残留的快速定量检测方法。结果试验结果表明，该方法对头孢氨苄的检测限为0．16ng／ml，半数抑制浓度（IC50）为0．6ng／ml，线性范围为0．16～5ng／ml，标准添加回收率为100％～115％之间，与头孢菌素类及青霉素等其他12种抗生素的交叉反应率均〈0．01％，与ELISA方法比较，测定结果相关性良好。结论本试验所建立的荧光免疫层析法快速检测牛奶中头孢氨苄残留，具有简便、快速、灵敏、直观的优点，可用于抗生素残留的筛查，极具推广和应用价值。Objective The cefalexin residues in milk immunochromatography assay and strip reader. Methods was rapidly and quantitatively detected by fluorescent The europium nanoparticles were synthesized by RP- microemulsion technology. The Eu nanoparticles conjugated with cefalexin monoclonal antibody was used as a fluorescent label. The cefalexin-ovalbumin and goat anti-mouse IgG was respectively used as the test line and control line on the fluorescent strip. A rapid and quantitative fluorescent immunoehromatography assay for detection of eefalexin residues in milk was established equipped with a strip reader. Results The fluorescent immunochromatography assay had a good correlation with ELISA, with the detection limit of O. 16 ng/mL, and the ICso value was 0. 6 ng/ml. The linear detection range was 0. 16-5 ng/ml. The recoveries of spiked milk were between 100%-115% , and the cross-reaction rate of the antibody with 12 kinds of antibiotics including eephalosporins and penicillin was less than 0.01%. Conclusion It was easy, rapid, and sensitive for the detection, and had the potential to be a powerful tool for screening other antibiotics residues in milk and milk products.

关 键 词：头孢氨苄 荧光免疫层析法 稀土铕纳米颗粒 快速定量检测 兽药残留 牛奶 抗菌素 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]