同时测定肉制品中酸性橙Ⅱ等11种合成色素的方法研究  

The method of simultaneous determination of 11 synthetic pigments including acid orange Ⅱ in meat products

作　　者：李静娜[1] 贺栋梁[2] 梁高道[1] 何振宇[1] 黄常刚[1] 陈曦[1] 周敦金[1] 

机构地区：[1]武汉市疾病预防控制中心,湖北武汉430015 [2]南华大学公共卫生学院,湖南衡阳421001

出　　处：《中国食品卫生杂志》2014年第4期372-376,共5页Chinese Journal of Food Hygiene

基　　金：武汉市卫生局资助课题(WG11B02)

摘　　要：目的构建同步测定肉制品中柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红、红色2G、亮蓝、酸性红、赤藓红、酸性橙Ⅱ等11种合成色素的高效液相色谱法（HPLC），对市售肉制品中色素进行监测。方法采用固相萃取技术，用乙醇氨水水溶液提取样品中色素并经WAX小柱（150mg，30μm）净化，采用高效液相色谱一二极管阵列检测器（HPLC-PDA）检测。结果HPLC法测定11种色素在1．00～20．0μg／ml范围内有较好的线性关系，r均〉0．999，方法检出限为0．02～0．06mg／kg，样品加标平均回收率为81．1％～100．8％，RSD为1．3％～4．9％（n=6）。用本方法监测259份市售肉制品，不同色素的检出率为0％～22．0％。结论方法具有较高的选择性和灵敏度，回收率和重现性良好，适用于肉制品中11种色素分析，具有推广应用价值。Objective To establish a high performance liquid chromatography （HPLC） method of simultaneous detection of 11 kinds of artificial pigments including Tartrazine, New red, Amaranth, Carmine, Sunset yellow, Allura Red, Red 2G, Acid blue 9, Azorubine, Erythrosine and Acid orange Ⅱ, et. al. Methods The target compounds in food samples were extracted by ethanol ammonia and purified by WAX cardge. The extracts were determined by a high performance liquid chromatography-diode array detector. Results The results showed that there were good linear relativities （ r 〉 0. 999） between artificial pigments and absorbance in the content ranging from 1.00 to 20. 0 μg/ml. The limit of detection, recovery rate and standard deviation were 0.02-0.06 mg/kg, 81. 1%-100.8% and 1.3%-4.9% , respectively （n = 6）. The detection rate of various pigments in 259 food samples ranged from 0%-22.0%. Conclusion The method was selective, sensitive and repeatable, and compatible for simultaneous detection of 11 kinds of artificial pigments.

关 键 词：高效液相色谱 合成色素 固相萃取 肉制品 违法添加 食品安全 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]