核桃花粉提取物的抗氧化性研究  

Study on the antioxidant activity of walnut pollen extracts

作　　者：王丹[1] 翟梅枝[1] 毛光瑞[1] 史冠昭[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《食品工业科技》2014年第16期137-139,144,共4页Science and Technology of Food Industry

基　　金：国家林业局<保健功能性核桃产业化开发技术研究>公益性行业专项(201004027);陕西省科技统筹创新工程计划(2012KTZB02-01-01)

摘　　要：以甲醇、乙醇、水为溶剂提取西林2、西林3、西扶2、西洛3和香玲等5个核桃品种花粉的活性成分,通过测定不同溶剂提取物的总酚含量、还原能力、DPPH自由基清除能力和抗脂质氧化能力评价核桃花粉的抗氧化活性。结果表明:核桃花粉提取物的还原能力与提取物中的总酚含量呈正相关,顺序为:甲醇提取物>乙醇提取物>水提取物;对DPPH自由基的清除作用大小为:乙醇提取物>甲醇提取物>水提取物;5种花粉的水提取物抗脂质氧化能力较强;西林2的还原能力和清除DPPH自由基的能力最强;西林3水提物的抗脂质氧化活性最好。The antioxidant properties of five different walnut pollen extracts （Xilin 2,Xilin 3,Xifu 2,Xiluo 3 and Xiangling） were assayed. The antioxidant activity of methanolic,ethanolic and water extracts were determined using different methods including reducing power, iron （II） chelation, DPPH radical scavenging, anti-lipid oxidation andthe total phenolic contents evaluation. The results showed that statistically significant relationship between the total phenolic content and the reducing power assay,iron（ll） chelation assay. The order was methanolic〉ethanolic〉water. The ability of DPPH radical was Ethanolic〉methanolic〉water. The water extracted from walnut staminate pollen showed higher TBA activities. Xilin 2 pollen extracts showed the highest scavenging activity for DPPH radicals and Xilin 3 water extracts showed the highest TBA activities.

关 键 词：核桃花粉 提取物 抗氧化性 

分 类 号：TS255.6[轻工技术与工程—农产品加工及贮藏工程]