卵巢浆液性肿瘤中Pax8和Tubulin的表达及意义  

Expression of Pax8 and Tubulin in ovarian serous tumors and their significances

作　　者：徐兵[1] 孙多祥[1] 康永军[1] 田园[1] 周颖[1] 冯定庆[1] 王志华[2] 陈珂[2] 胡卫平[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科,合肥230001 [2]安徽医科大学附属省立医院病理科,合肥230001

出　　处：《临床与实验病理学杂志》2014年第7期713-716,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81001168;81272881);安徽省2014年度重点立项项目(1301043053)

摘　　要：目的探讨Pax8和Tubulin在卵巢浆液性肿瘤中的表达及意义。方法采用免疫组化SP法检测28例正常卵巢组织、38例卵巢良性浆液性囊腺瘤、34例卵巢交界性浆液性肿瘤和38例卵巢浆液性癌中Pax8、Tubulin的表达,并复习相关文献。结果正常卵巢上皮中Pax8、Tubulin均呈阴性,卵巢良性浆液性囊腺瘤、交界性浆液性肿瘤、浆液性癌中Pax8、Tubulin阳性率分别为78.9%和78.9%、100%和100%、94.7%和89.5%,Pax8在卵巢交界性浆液性肿瘤及浆液性癌组织中表达明显高于良性浆液性囊腺瘤组织(P<0.05),Tubulin在3种卵巢肿瘤中的表达差异无显著性(P>0.05),但存在表达部位的改变;Pax8表达与卵巢浆液性癌患者年龄、FIGO分期、病理学分级、淋巴结转移无关(P>0.05),Tubulin表达与浆液性癌病理学分级有关(P<0.05),Pax8、Tubulin的免疫组化评分比值大小与淋巴结转移密切相关(P<0.05)。结论 Pax8可能作为卵巢浆液性癌的标志分子,并为其临床靶向分子治疗提供实验依据;综合分析Pax8、Tubulin及二者免疫组化评分比值,对评估卵巢浆液性癌患者的病理学分级、淋巴结转移情况具有参考意义。Purpose To investigate the expression of Pax8 and Tubulin protein in ovarian serous tumors and their clinicopathologic significances. Methods Immuohistochemistry was used to detect the expressions of Pax8 and Tubulin protein in 28 normal ovarian epithelium, 38 ovarian serous cystadenoma, 34 ovarian serous borderline tumor, 38 ovarian serous cancer samples and the relevant literature is reviewed. Results Pax8 and Tubulin in normal ovarian epithelium were not expressed, while the positive rates of Pax8 and Tubulin in ovarian serous cystadenoma, ovarian serous borderline tumor, ovarian serous cancer samples were 78.9% and 78.9% , 100% and 100%, 94. 7% and 89.5% , respectively. Immunohistochemical staining was scored which shows the expression of Pax8 in ovarian serous borderline tumor, ovarian serous cancer samples is significantly higher than ovarian serous cystadenoma （ P 〈 0. 05 ） , and Tubulin in the three ovarian tumors is not different （ P 〉 0. 05 ） , but there is a change of the expression sites of Tubulin. The expression level of Pax8 is not associated with the age of ovarian serous cancer patients, FIGO stage, pathological grade, lymph node metastasis （P 〉 0. 05 ） , Tubulin expression is correlated with pathological grade （ P 〈 0. 05 ）. The mmunohistochemical scores ratio of Pax8 and Tubulin is related to lymph node metastasis （ P 〈 0. 05 ）. Conclusion Pax8 expression may be ＂used as a molecular marker and clinical treatment target of ovarian serous cancer. There is an important reference value to assess the histological grade, lymph node metastasis of ovarain serous cancer patients by comprehensive analysis of Pax8, Tubulin and the ratio of Pax8 and Tubulin.

关 键 词：卵巢肿瘤 浆液性癌 分泌细胞 纤毛细胞 Pax8 TUBULIN 

分 类 号：R737.9[医药卫生—肿瘤]