血管紧张素转换酶基因多态性与结直肠癌的相关性  

Relationship Between Polymorphism of AngiotensinⅠ-Converting Enzyme Gene and Colorectal Carcinoma

作　　者：侯安继[1] 胡艳[1] 赵德民[2] 茅佩娟[3] 高红芳[1] 周莉[1] 

机构地区：[1]上海市第七人民医院肿瘤科,上海200137 [2]上海市第七人民医院外科,上海200137 [3]上海市第七人民医院心内科,上海200137

出　　处：《武汉大学学报（医学版）》2014年第4期560-564,共5页Medical Journal of Wuhan University

基　　金：上海市卫生局资助项目(编号:20114196);上海市中医临床重点学科基金资助项目(编号:ZYXK2012010)

摘　　要：目的:探讨血管紧张素转换酶(ACE)基因多态性在结直肠癌(CRC)发病中的作用。方法:入组120例CRC患者,采用聚合酶链反应(PCR)检测患者外周血中ACE基因多态性和等位基因分布频率,研究ACE基因缺失型(DD型)、II型和插入型(ID型)等3种基因型及I/D等位基因在CRC患者外周血中的分布频率,并与120例正常人检测结果进行对比。结果:CRC患者外周血标本中存在ACE基因插入/缺失(I/D)多态性分布,DD型、ID型和II型3种基因型的频率分别为15.00%,50.00%,35.00%,I/D等位基因分布频率分别为60.00%及40.00%;对照组的分布频率分别为16.67%,53.33%,30.00%,I/D等位基因分布频率分别为56.67%及43.33%,两组间3种基因型的分布频率无显著性差异(P>0.05)、I/D等位基因分布频率无显著差异(P>0.05)。分层分析表明,D等位基因变异与CRC低分化和远处转移相关(P<0.01及P<0.05)。结论:ACE基因I/D多态性与CRC存在一定关系,其中D等位基因异常与CRC低分化和易转移可能有关。Objective： To observe the correlation between the insertion/deletion （I/D） polymorphism of angiotensin Ⅰ-converting enzyme gene （ACE） and colorectal cancer （CRC）. Methods： 120 CRC patients were enrolled in the study. Polymerase chain reaction （PCR） was served to detect insertion/deletion genotypes and I/D alleles of ACE gene in peripheral blood, and 120 volunteers served as control. Results： In CRC patients, the frequencies of genotypes of DD, ID and II of ACE gene were 15.00%, 50.00%, 35.00% respectively, I/D alleles frequencies were 60.00% and 40.00% respectively;in the control, the frequencies of genotypes of DD, ID and II of ACE gene were 16. 67%, 53. 33%, 30.00%, respectively, I/D alleles frequencies were 56. 67% and 43.33% respectively. The distribution of DD, ID and II genotypes of ACE gene and I/D alleles between the two groups showed no significant difference （P〉0.05）. However, by stratification analyses, it was found that the change of D allele was correlated with poor differentiation and metastasis （P〈0.01 and P〈0.05）. Conclusion ： There was a correlation between ACE gene I/Dpolymorphism and CRC, and D allele might be a factor of poor differentiation and metastasis of CRC patients.

关 键 词：结直肠癌 血管紧张素转换酶 等位基因 多态性 

分 类 号：R735.34[医药卫生—肿瘤]