猪痢疾短螺旋体荧光PCR检测方法的建立与初步应用  

Development and Application of TaqMan Real-time PCR for Brachyspira hyodysenteriae

作　　者：张伟[1] 高志强[1] 李宁[2] 谷强[1] 凌凤俊[1] 乔彩霞[1] 蒲静[1] 张利峰[1] 汪琳[1] 吴丹[1] 柏亚铎[1] 安健[3] 刘环[1] 赖平安[1] 张鹤晓[1] 

机构地区：[1]北京出入境检验检疫局,北京通州101113 [2]中国兽医药品监察所,北京海淀100081 [3]北京农学院,北京昌平102206

出　　处：《中国兽医杂志》2014年第6期9-11,14,共4页Chinese Journal of Veterinary Medicine

基　　金：国家质量监督检验检疫总局科技计划(2008IK028)

摘　　要：在对设计合成的引物和6-carboxy-fluorescein(6-FAM)荧光素标记的Taq Man水解探针进行筛选的基础上,通过反应体系优化,建立了猪痢疾短螺旋体荧光PCR检测方法。特异性、敏感性、重复性及临床样品的检测结果均表明建立的方法快速、特异、灵敏,整个检测过程(包括样品的前处理时间)可在4 h内完成。建立的方法大大缩短了该病原的检测周期,并为临床诊断提供快速、敏感、特异性强的检测方法。Based on the screening of the designed primers and TaqMan hydrolysis type probes labeled with 6-carboxy-fluorescein（6-FAM） reporter dyes,this research developed a real-time PCR detection method for Brachyspira hyodysenteriae by means of optimazation of reaction conditions.The sensitivity,specificity,repeatability and detection clinical samples of the real-time PCR were examined respectively.The results showed that the method were rapid,specific and sensitive.The assays could be finished in 4 hours（including the pretreatment of samples）.The developed method shortens the detecting period of swine dysentery,and at the same time it provides a rapid,sensitive and specific method for clinical diagnosis.

关 键 词：猪痢疾 猪痢疾短螺旋体 荧光PCR 

分 类 号：S852.651[农业科学—基础兽医学]