河南地区汉族人群原因不明复发性流产与HLA-DRB1等位基因多态性的相关性  

Association of polymorphisms of HLA-DRB1 gene with unexplained recurrent spontaneous abortion in ethnic Hans from Henan

作　　者：何淼[1] 康冰[1] 廖世秀[1] 杨科[1] 丁雪冰[1] 吴东[1] 郭谦楠[1] 侯巧芳[1] 

机构地区：[1]郑州大学人民医院,河南省人民医院河南省,医学遗传研究所,450003

出　　处：《中华医学遗传学杂志》2014年第4期504-507,共4页Chinese Journal of Medical Genetics

基　　金：河南省医学科技攻关计划普通项目（201303162）

摘　　要：目的探讨河南地区汉族人群人类白细胞抗原（human leukocyte antigen，HLA）DRB1区域基因多态性的分布及与原因不明复发性流产（unexplained recurrent spontaneous abortion，URSA）易感性的关系。方法应用序列特异性引物聚合酶链反应分析200对URSA夫妇（患者组）和200对无不良妊娠史夫妇（对照组）的DRB1基因型。结果（1）患者组DRB1＊09及DRB1＊13等位基因频率显著高于对照组（14．50％ vs．9．50％，7．00％佛．4．38％，均P〈0．05），DRB1＊04及DRB1＊12等位基因频率较对照组显著降低（7．13％ vs．10．75％，8．63％ vs．14．38％，均P〈0．05）。（2）患者组中女性DRB1＊09（14．00％）等位基因频率显著高于对照组女性（9．25％）（P=0．036），DRB1＊12（8．50％）等位基因频率较对照组女性显著降低（14．00％）（P=0．014）。（3）患者组中男性DRB1＊09及DRB1＊13等位基因频率显著高于对照组男性（15．00％ vs．9．75％，9．25％ vs．4．00％，均P〈0．05），DRB1＊04及DRB1＊12等位基因频率较对照组男性显著降低（5．75％ vs．12．25％，8．75％ vs．14．75％，均P〈0．05）。结论河南地区汉族人群中DRB1＊09及DRB1＊13可能是URSA的易感基因，而DRB1＊04及DRB1＊12可能对URSA的发生有保护作用；但是在患者组女性中，DRB1＊13及DRB1＊04位点与URSA的相关性不明显。Objective To assess the association of polymorphisms of human leukocyte antigen DRB1 gene （HLA-DRB1） with susceptibility to unexplained recurrent spontaneous abortion （URSA）. Methods The HLA-DRB1 gene was typed with polymerase chain reaction-specific sequence primers （PCR-SSP） method in 200 couples with URSA and 200 couples with a normal pregnancy history. Results The frequencies of DRB1 ＊ 09 and DRB1 ＊ 13 alleles were significantly greater in the URSA group compared with the control group （14.50% vs. 9.50%, and 7.00% vs. 4.38%, both P〈0.05）, whilst the frequencies of DRB1 ＊ 04 and DRB1＊ 12 alleles were significantly lower （7.13% vs. 10.75%, and 8.63% vs. 14.38%, both P〈0.05）. For females from the URSA group, the frequency of DRB1 ＊ 09 allele （14.00%） was significantly higher compared with the controls （9.25%） （P=0. 036）, whilst the frequency of DRB1＊ 12 （8.50%） allele was significantly lower （14. 00%） （P= 0.014）. For males in the URSA group, the frequencies of DRB1 ＊ 09 and DRB1＊ 13 alleles were significantly higher than those of the controls （15.00% vs. 9.75%, and 9.25% vs. 4.00%, both P〈0.05）, whilst the frequencies of DRB1＊ 04 and DRB1＊ 12 alleles were significantly lower （5. 75% vs. 12. 25%, and 8. 75% vs. 14. 75%, P〈0.05）. Conclusion The DRB1＊ 09 and DRB1 ＊ 13 alleles may contribute to the susceptibility of URSA, while DRB1 ＊ 04 and DRB1 ＊ 12 alleles may confer a protective effect factors. For females, however, no significant association of DRB1＊ 13 and DRB1＊ 04 alleles with URSA was found.

关 键 词：人类白细胞抗原 原因不明复发性流产 等位基因频率 多态性 

分 类 号：R714.21[医药卫生—妇产科学]