可切除肺腺癌组织中甲状腺转录因子1表达与表皮生长因子受体基因突变的关系  

Correlation between Expression of Thyroid Transcription Factor 1 and Gene Mutation of Epidermal Growth Factor Receptor in Patients with Resectable Lung Adenocarcinoma

作　　者：林海平[1] 曹子昂[1] 杨异[2] 叶清[1] 

机构地区：[1]上海交通大学医学院附属仁济医院胸外科,上海200127 [2]上海市第六人民医院心胸外科,上海200233

出　　处：《中国胸心血管外科临床杂志》2014年第4期518-521,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的探讨可切除肺腺癌组织中甲状腺转录因子1（TTF-1）表达与表皮生长因子受体（EGFR）基因突变的关系。方法纳入2009年6月至2012年12月在上海市第六人民医院心胸外科及上海交通大学医学院附属仁济医院胸外科接受肺癌手术后病理证实为肺腺癌的患者67例,男40例、女27例,年龄37~79（56.7±1.8）岁。针对术后肿瘤标本,分别应用免疫组织化学方法检测TTF-1表达,突变富集体聚合酶链反应（PCR）法检测EGFR基因突变。应用校正卡方检验进行统计学分析。结果 67例肺腺癌组织中TTF-1阳性57例,阴性10例;EGFR基因突变者44例。TTF-1阳性患者中EGFR基因突变率为73.7%（42/57）,TTF-1阴性患者中EGFR基因突变率为20.0%（2/10）。以TTF-1表达预测EGFR突变的敏感度为95.5%,特异度为34.8%。结论肺腺癌中TTF-1阳性表达患者具有更高的EGFR突变可能,肺腺癌中TTF-1阳性表达对EGFR基因突变具有预测价值。Objective To explore the correlation between expression of thyroid transcription factor-1 （TTF-1 ） and gene mutation of epidermal growth factor receptor （EGFR） in patients with resectable lung adenocarcinoma （LAC）. Method Sixty-seven LAC patients who underwent surgical resection in the Department of Cardiothoracic Surgery of Shanghai No.6 Hospital and Department of Thoracic Surgery, Renji Hospital, School of Medicine of Shanghai Jiaotong University from June 2009 to December 2012 were enrolled in this study. There were 40 male and 27 female patients with their age of 37- 79 （56.7 ± 1.8 ） years. TTF- 1 expression was detected by immunohistochemistry. EGFR gone mutation was examined with mutant-enriched polymerase chain reaction. The correlation between TTF-1 expression and EGFR gone mutation was analyzed with corrected chi-square test. Results Among the 67 LAC samples, 57 samples were TTF-1 positive and 10 samples were TTF-1 negative. There was EGFR gone mutation in 44 samples. EGFR gone mutation rate was 73.7% （42/57） in TTF- 1 positive patients and 20.0% （2/10） in TTF- 1 negative patients. The sensitivity of TTF- 1 expression to predict EGFR mutation was 95.5%, and the specificity was 34.8%. Conclusion EGFR gone mutation rate is higher in LAC patients with positive TTF-1 expression. Positive TTF-1 expression can be used to predict EGFR gone mutation in LAC patients.

关 键 词：肺癌 腺癌 甲状腺转录因子1 表皮生长因子受体 基因突变 

分 类 号：R734.2[医药卫生—肿瘤]