人工合成小麦HMW-GS D^tx1.5亚基的AS-PCR鉴定程序优化及遗传分析  

Optimization of AS-PCR Identification System for HMW-GS D^tx1.5 of Wheat Syndeme Developed with Durum Wheat and Aegilops tauschii and Its Genetic Analysis

作　　者：万洪深 温雯[2] 李俊 杨武云 

机构地区：[1]四川省农科院作物研究所农业部西南地区小麦生物学与遗传育种重点实验室,成都610066 [2]农业部科技发展中心,北京100122

出　　处：《分子植物育种》2014年第4期629-637,共9页Molecular Plant Breeding

基　　金：国家小麦产业技术体系(CARS-3-2-41);四川省科技支撑项目(2011NZ0098-16);四川省科技计划项目(国际合作)(2010HH0052)共同资助

摘　　要：人工合成小麦综合了自然界中现有四倍体小麦(Triticum turgidun)和粗山羊草(节节麦,Aegilops tauschii)的丰富遗传变异,是将野生祖先种中优异的抗病、抗逆基因成功利用到普通小麦育种中的重要桥梁。源于粗山羊草的高分子量谷蛋白(HMW-GS)Dtx1.5亚基,是一种新的优质亚基。本研究对HMW-GSDtx1.5亚基AS-PCR反应体系及扩增程序进行了逐步的优化,并利用普通小麦川育12与人工合成小麦Syn780(含有Dtx1.5亚基)杂交F2代(低代)和F9重组自交系(高代)群体对Dtx1.5亚基的遗传规律进行了分析。结果表明:优化后目的扩增条带清晰、稳定、可靠,可以用于Dtx1.5亚基的分子鉴定;Dtx1.5亚基在普通小麦与人工合成小麦杂交的低代和高代群体中的分布都符合孟德尔分离定律,说明源于粗山羊草的Dtx1.5亚基能在小麦遗传背景下遗传稳定,传递正常,可用于小麦品质育种。Synthetic hexaploid s have gradually been used as a bridge-tool for common wheat improvement to introduce elite gene sources from its wild relatives with strong resistance to biotic/abiotic stresses, increased yield potential and good making quality. The high molecular weight glutenin subunits（HMW-GS） Dtx1.5 from Aegilops tauschii is thought to be associated with fine making quality. In this study, the reaction condition of the Dtx1.5AS-PCR system has been optimized step by step, and the distribution of the HMW-GS Dtx1.5 in the F2 and F9RIL populations derived from Chuanyu 12 crossed with synthetic wheat Syn780 was also investigated using this AS-PCR system. The sharp and stable fragments amplified by this optimum AS-PCR system indicated its availability and convenience in the identification of HMW-GS Dtx1.5. And the observed distribution of Dtx1.5obeyed Mendelian law of segregation in both low（F2） and advanced（F9） generation populations, suggesting the stable inheritance of Dtx1.5. Therefore, the Dtx1.5 from Aegilops tauschii could be used in common wheat breeding for quality and the optimum AS-PCR system could enhance the efficiency of selection by molecular markers.

关 键 词：人工合成小麦 Dtx1 5亚基 AS-PCR 遗传分析 

分 类 号：S512.1[农业科学—作物学]