Hydrolytic Enzyme Inhibitors and Necrotic Reactions in Potato Leaves Reduce Reproductive Success of Colorado Potato Beetle  

作　　者：Rinat Ismagilovich Ibragimov Ildar Salimyanovich Mardanshin Galina Vasilyevna Benkovskaya Maksim Borisovich Udalov Irina Andreevna Shpirnaya Vyacheslav Olegovich Tsvetkov 

机构地区：[1]Department of Biology, Bashkir State University, City of Ufa 450076, Republic of Bashkortostan, Russia [2]Laboratory of Potato Research, Bashkir Scientific-Research Institute of Russian Agricultural Academy, City of Ufa 450059, Republic of Bashkortostan, Russia [3]Laboratory of lnsects Adaptivity Biochemistry, Institute of Biochemistry and Genetics USC RAS, City of Ufa 450095, Republic of Bashkortostan, Russia

出　　处：《Journal of Agricultural Science and Technology(A)》2014年第4期331-341,共11页农业科学与技术（A）

摘　　要：In field and laboratory conditions the reproductive potential （survival at different stages of development） of the Colorado potato beetle has been measured, the dependence of the parameters of insects fertility on varietal features of the feed has been investigated, the food activity of the representatives of insects from four areas of the Southern Urals has been measured, the intensity and time of hypersensitivity reactions on potato leaves has been defined. In laboratory experiments, the level of activity of hydrolytic enzymes in insects and the level of activity of these enzymes inhibitors in the leaves of three potato varieties differing in their resistance to the Colorado potato beetle have been determined. The results show that the insects-representatives of different samples have different food activity in relation to different potato varieties. High mortality rates were revealed at feeding with leaves of potato of the Bashkir variety characterized by a high level of hydrolases inhibitors and relatively rapid development of necrotic reactions after the appearance of eggs laying on the leaves. We suppose that the neerogenetic barrier appearing in the form of necrosis is the major cause of the fetal mortality of insects. The level of the content of inhibitors inactivating larvae digestive enzymes affects the survival of insects at postembryonic stages. The speed of the necrosis emergence, the level of activity of digestive enzymes inhibitors in potato can be considered as criteria for the selection of breeding material for resistance to the Colorado potato beetle.

关 键 词：Potato Colorado beetle necrogenetic barrier PROTEASE AMYLASE PECTINASE cellulose hydrolases inhibitors. 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治] TQ465.92[农业科学—植物保护]