丙酮磷酸氢二钾双水相体系提纯黄褐毛忍冬中的绿原酸  

Separation and purification of chlorogenie acid from Lonicera fulvotomentosa with aqueous two-phase systems containing acetone and Dipotassium Hydrogen Phsophate

作　　者：何磊磊[1] 玉秋萍[1] 张妮[1] 王家胜[1] 孔琪[1] 张齐胜[1] 余正文[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001 [2]贵州师范大学贵州省植物生理与发育重点实验室,贵州贵阳550001

出　　处：《食品工业科技》2014年第17期198-200,205,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(31060056);贵州师范大学博士科研项目;贵州省中药现代化专项(黔科合中药字[2011]5046号);贵州省科技基金项目(黔科合J字[2011]2368号);贵州省科技创新人才团队建设项目(黔科合人才团队[2009]4007号)

摘　　要：本研究采用丙酮-磷酸氢二钾双水相体系法提纯黄褐毛忍冬中的绿原酸,通过正交实验测定了酮水比、盐液比和料液比对绿原酸萃取率及提取物纯度的影响。结果表明:按酮水比8.5∶1.5(V/V),盐液比为100∶1(mg/mL)配制双水相,料液比25∶1(mg/mL),绿原酸的萃取率为91.10%,纯度从原料中的20.16%提高到42.13%。该工艺溶剂易回收、易处理、操作简便,适于黄褐毛忍冬中绿原酸的提纯。Chlorogenie acid was separated and purified from Lonicera fulvotomentosa crude extract by acetone- K2HPQ two-phase aqueous system.Influence of material/liquid ratio, acetone/water ratio and salt/liquid ratio on extraction rate and purity of chlorogenie acid was investigated by orthogonal test. The results showed that extraction yield of chlorogenie acid was 91.10% under acetone-water ratio for 8.5：1.5 （V/V）, salt-solvent ratio for 100：1 （mg/mL） and material-solvent ratio for 25：1（mg/mL） ,the purity of chlorogenie acid increases from 20.16% with crude extract 42.13%. This method was easy to recovered solvent, dispose and operate, which fit for purification of chlorogenie acid from Lonicera fulvotomentosa.

关 键 词：丙酮-磷酸氢二钾 双水相体系 黄褐毛忍冬 绿原酸 

分 类 号：TS201.1[轻工技术与工程—食品科学]