超高效液相色谱法同时测定食品中7种合成色素  

Determination of Seven Synthetic Pigments in Foods by Ultra Performance Liquid Chromatography

作　　者：鄢兵[1] 胡海山[1] 张云伟[1] 赵淑娥[1] 芦慧[1] 

机构地区：[1]江西省分析测试研究所,江西南昌330029

出　　处：《分析科学学报》2014年第4期525-528,共4页Journal of Analytical Science

摘　　要：建立了超高效液相色谱法（UPLC）同时测定食品中柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红和亮蓝7种人工合成色素。采用ACQUITY UPLBEHC18柱（50×2.1mm,1.7μm）,以0.02mol/L的乙酸铵和甲醇为流动相,梯度洗脱,流速0.3mL/min,柱温30℃,检测波长为254nm和628nm,用外标法定量。7种合成色素在1～100μg/mL范围有良好的线性关系（r≥0.9994）,平均回收率在83.5%～99.9%范围,相对标准偏差为0.57%～2.91%。该法简便、快速、灵敏,结果准确可靠,适用于食品中上述7种人工合成色素的分析。An ultra performance liquid chromatography （UPLC） method for the simultaneous determination of seven synthetic pigments in foods was established in this paper. The samples were separated on a Waters ACQUITY UPLC BEH C18 column（50×2.1mm,1.7μm） with the mobile phase of methanol and 0.02 mol/L ammonium acetate for gradient elution. The flow rate was 0.3 mL/min, column temperature was 30 ℃ and detection wavelength was 254 nm and 628 nm. The content was determined by external standard method. The seven synthetic pigments exhibited excellent linearity over a range of 1～100μg/mL（r≥0. 9994）. The average recoveries were between 83.5% and 99.90%, and the relative standard deviations were between 0.57 % and 2.91%. This method is simple,rapid, accurate and reliable, which is suitable for the determination of synthetic pigments in foods.

关 键 词：超高效液相色谱 食品 合成色素 

分 类 号：O657.72[理学—分析化学]