大肠癌转移和复发与KRAS及磷脂酰肌醇-3-激酶催化亚单位α基因突变的关系  

Correlation betwwen mutations of KRAS/Phosphatidylinositol 3-kinase catalytic alpha with metastasis and recurrence of colorectal cancer

作　　者：范华骏 郑勇斌[1] 童仕伦[1] 

机构地区：[1]武汉大学人民医院胃肠外科,430060

出　　处：《中华实验外科杂志》2014年第8期1819-1821,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 检测大肠癌组织中KRAS与磷脂酰肌醇-3-激酶催化亚单位oα（PIK3CA）突变,探讨其与大肠癌转移、复发之间的关系.方法 收集大肠癌标本94例,提取DNA后行KRAS和PIK3CA基因测序,分析其临床病理特征,探讨两者之间的关系;并对患者进行为期3年的随访,分析基因突变与转移、复发的关系.结果 KRAS基因突变31例,远处转移率高于野生型（61.3％比34.9％）;PIK3CA基因突变15例,远处转移率高于野生型（73.3％比38.0％）;两者共同突变7例,均未突变55例,共同突变者转移率高于均未突变者（71.4％比29.1％）;术后3年随访KRAS基因突变型转移率和复发率均高于野生型（93.1％比69.5％,65.5％比39.0％）,PIK3CA基因突变型转移率和复发率亦均高于野生型（93.3％比74.0％,73.3％比42.5％）;上述结果差异均有统计学意义（P＜0.05）.结论 大肠癌组织中KRAS和PIK3CA基因突变型远处转移率和复发率高,对大肠癌的远处转移和复发可能有一定的预测价值.Objective To detect KRAS and Phosphatidylinositol 3-kinase catalytic alpha （PIK3CA） gene mutations in colorectal cancer,and to assess their relationships with metastasis and relapse.Methods Ninety-four CRC tumor specimens were investigated,and KRAS/PIK3CA mutations were detected by gene sequencing analysis after genomic DNA extracted.The mutation results were analyzed with the corresponding clinical pathological data,especially with metastasis,and 3-year follow up data were also obtained for metastasis and recurrence.Results Thirty-one （33.0％） had KRAS gene mutations,and there was an increased incidence of metastasis with KRAS mutant when compared with wild-type （61.3％ vs.34.9％）.Fifteen （16.0％） had PIK3CA gene mutations,and there was also an increased incidence of metastasis as compared with wild-type （73.3％ vs.38.0％）.Seven cases （7.4％） had both KRAS and PIK3CA mutations,and displayed an increased metastasis when compared with neither KRAS nor PIK3CA mutations.Three-year follow-up data demonstrated an increased incidence of metastasis and recurrence with KRAS mutant （93.1％ vs.69.5％,and 65.5％ vs.39.0％）,and PIK3CA showed the same trend （93.3％ vs.74.0％,and 73.3％ vs.42.5％）,P＜0.05.Conclusion KRAS and PIK3CA mutants were related with metastasis and recurrence,and may have a predictive value for metastasis and recurrence.

关 键 词：大肠癌 转移 KRAS基因 磷脂酰肌醇-3-激酶催化亚单位α基因 基因突变 

分 类 号：R735.34[医药卫生—肿瘤]