木薯粉同步糖化发酵(SSF)产丁二酸  

Succinic acid fermentation by simultaneous saccharification and fermentation(SSF) with cassava flour

作　　者：申乃坤[1,2] 王青艳[1,2] 秦艳[1,2] 廖思明[1,2] 朱婧[1] 朱绮霞[1] 米慧芝[1] 黄日波[1,2] 

机构地区：[1]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物炼制重点实验室,广西南宁530007 [2]广西大学生命科学与技术学院广西亚热带生物资源保护利用重点实验室,广西南宁530005

出　　处：《微生物学通报》2014年第8期1507-1515,共9页Microbiology China

基　　金：国家自然科学基金项目(No.31160023);广西自然科学基金项目(No.2013GXNSFBA019102);广西科学院基金项目(No.13YJ22SW);八桂学者建设工程专项经费

摘　　要：【目的】通过优化产琥珀酸放线杆菌GXAS137同步糖化发酵木薯粉产丁二酸的发酵培养基,提高丁二酸产量,降低生产成本。【方法】在单因素试验的基础上,先利用Plackett-Burman试验设计筛选出影响丁二酸发酵的重要参数,再采用正交试验确定重要参数的最佳水平。【结果】价格低廉玉米浆可用作氮源,影响丁二酸产量的重要参数是木薯粉、玉米浆、碱式碳酸镁和糖化酶浓度。最佳条件为(g/L):木薯粉100,玉米浆14,糖化酶2.0 AGU/g底物,碱式碳酸镁75。优化后丁二酸产量达到69.31 g/L,丁二酸得率为90.01%,生产强度为1.44 g/(L·h)。与初始条件(52.34 g/L)相比,丁二酸浓度提高了32.42%。并利用1.3 L发酵罐对SSF与SHF两种发酵工艺进行了比较,SSF丁二酸产量(72.21 g/L)远高于SHF(56.86 g/L)。【结论】产琥珀酸放线杆菌同步糖化发酵木薯粉丁二酸产量高,生产成本低,具有较好的工业化应用前景。[Objective] In order to succinic acid production from raising yields Actinobacillus and reducing production costs, succinogenes GXAS 137 by we optimized simultaneous saccharification and fermentation （SSF） with cassava flour as carbon source. [Methods] The important parameters were screened by the single factor experiment and Plackeet-Burman design. Then, the optimum values of the parameters were obtained by orthogonal experiment design. [Results] The results showed that corn steep liquor （CSL） was used as a proper nutrient in the succinic acid production from cassava flour. The optimum medium compositions were determined as （g/L）： cassava flour 100, CSL 14, glucoamylase dose 2.0 AGU/g substrate and MgCO3 75. The succinic acid yield reached 69.31 g/L at the optimal condition. The conversion rate of cassava to succinic acid reached 90.01% and succinic acid productivity was 1.44 g/（L.h）. The succinic acid content increased by 32.42% than that without the optimized condition （56.86 g/L）. The simultaneous saccharification fermentation （SSF） and separated hydrolysis and fermentation （SHF） were compared with 1.3 L fermenter. The results showed that the succinic acid of SSF （72.21 g/L） was superior than SHF. [Conclusion] The high-level succinic acid production and low production cost from cassava flour may facilitate industrial scale application in future.

关 键 词：产琥珀酸放线杆菌 木薯粉 Plackett-Burman试验 正交试验 丁二酸 

分 类 号：TQ921[轻工技术与工程—发酵工程]