Urantide对急性肝衰竭小鼠肝组织中IRF3表达的影响  

Urantide inhibits hepatic IRF3 expression in acute liver failure mice

作　　者：汪小庭 涂文娟[1] 刘亮明[1,2] 梁冬雨[2] 于芳苹[1] 赵亮[1] 叶长根[2] 杨志文[2] 高得勇[2] 

机构地区：[1]南京医科大学附属上海松江中心医院感染科,上海市201600 [2]上海交通大学附属第一人民医院松江分院感染科,上海市201600

出　　处：《世界华人消化杂志》2014年第18期2559-2564,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81070357;30660066~~

摘　　要：目的:探讨urotensinⅡ(UⅡ)受体拮抗剂urantide对急性肝衰竭小鼠肝组织干扰素调节因子3(interferon regulatory factor 3,IRF3)表达的影响.方法:♂Balb/c小鼠随机分为4组(每组6只).A组:健康对照组;B组:预处理对照组;C组:模型组;D组:预处理模型组.预处理组给予0.6 mg/kg urantide尾静脉注射.30 min后模型(包括预处理模型)组立即以脂多糖(lipopolysaccharide,LPS)联合D-半乳糖胺(D-galactosamin,D-GalN)腹腔注射诱导急性肝衰竭.LPS/D-GalN攻击12 h后采集小鼠肝组织标本.采用RT-PCR和实时荧光定量PCR(Real-time PCR)检测小鼠肝组织中IRF3mRNA表达水平,免疫印迹(Western blot)检测IRF3蛋白表达含量.结果:C组小鼠肝组织中IRF3 mRNA经RTPCR和Real-time PCR检测,其相对表达水平显著高于A组和B组(均P<0.001),D组显著低于C组(均P<0.001),而A和B组之间无明显差异(P>0.05);C组IRF3蛋白表达水平显著高于A组和B组(均P<0.001),D组显著低于C组(P<0.001),而A和B组之间无明显差异(P>0.05).结论:UⅡ受体特异性拮抗剂urantide可抑制LPS/D-GalN诱导ALF小鼠肝组织IRF3 mRNA和蛋白质表达.AIM： To investigate the effect of urantide, a urotensin Ⅱ（UⅡ） receptor inhibitor, on interferon regulatory factor 3（IRF3） expression in the liver tissue of mice with acute liver failure（ALF）. METHODS： Male Balb/c mice were randomly divided into four groups（n = 6 for each group）： normal control, pre-treatment control, model and pre-treatment model. The pre-treatment mice received urantide（0.6 mg/kg body weight） via a caudal vein injection. At 30 min post-injec-tion, the model（including pre-treatment model） mice were treated with lipopolysaccharide（LPS）/D-galactosamine（D-GalN） to induce ALF via an intraperitoneal injection. Liver tissues were sampled 12 h after LPS/D-GalN injection. IRF3 mRNA expression was detected by RT-PCR and real-time polymerase chain reaction（PCR）, and protein expression was detected by Western blot assay. RESULTS： The relative levels of IRF3 mRNA were significantly higher in model mice than in control and pretreatment control mice（P〈0.001 for all）. Compared with the model group, pretreatment model mice had significant lower levels of IRF3 mRNA（P〈0.001）. IRF3 protein levels were also significantly higher in model mice than in control and pretreatment control mice（P〈0.001 for all）, while the protein levels were significantly lower in pretreatment model mice than in model mice（P〈0.05）. CONCLUSION： Urantide can inhibit the up-regulation of IRF3 mRNA and protein expression in the liver tissue of mice with LPS/D-GalNinduced ALF.

关 键 词：急性肝衰竭 URANTIDE 干扰素调节因子3 小鼠 

分 类 号：R575.3[医药卫生—消化系统]