五味子乙素对人卵巢癌Skov3细胞株的增殖、凋亡及Wnt／β-catenin信号通路的影响  

Influences of Schisandrin B on proliferation,apoptosis and Wnt /β-catenin signaling pathway of Skov3 human ovarian cancer cells

作　　者：曾雯琼 徐青[2] 许银燕[3] 

机构地区：[1]江苏淮安淮安市妇幼保健院妇产科,223002 [2]南京医科大学附属南京妇幼保健院妇科,210004 [3]南京医科大学附属南京妇幼保健药学部,210004

出　　处：《临床肿瘤学杂志》2014年第7期589-593,共5页Chinese Clinical Oncology

摘　　要：目的探讨五味子乙素(Sch B)对人卵巢癌Skov3细胞株的增殖、凋亡及Wnt/β-catenin信号通路的影响。方法采用0、1.0、10.0、20.0、50.0μmol/L Sch B处理Skov3细胞,采用四甲基偶氮唑盐(MTT)法检测各浓度处理24、48、72和96h的增殖抑制率,Annexin-FITC/PI双染法检测不同浓度处理48h后的细胞凋亡情况,流式细胞仪检测各浓度处理48h后的细胞周期分布情况,Western blotting检测各浓度处理48h后细胞核Wnt/β-catenin信号通路中β-连接素(β-catenin)及下游靶分子C-myc、细胞周期素D1(Cyclin D1)的蛋白水平,同时于各浓度处理48h后检测细胞质和细胞核的糖原合成酶激酶-3β(GSK-3β)活性。结果 Sch B可呈剂量和时间依赖方式增加细胞增殖抑制率(P<0.05),作用48h后可呈剂量依赖方式升高早、晚期凋亡率及细胞质/胞核GSK-3β活性(P<0.05);除1.0μmol/L外,其余浓度作用48h的G0/G1期细胞比例均高于0μmol/L,S期、G2/M期细胞比例及β-catenin、C-myc和Cyclin D1蛋白水平均低于0μmol/L(P<0.05),且10.0、20.0、50.0μmol/L Sch B间的差异有统计学意义(P<0.05)。结论 Sch B可以抑制Skov3细胞株的增殖并促进其凋亡和细胞周期阻滞,以及抑制Wnt/β-catenin通路的激活。Objective To explore the influences of Schisandrin B （ Sch B） on proliferation, apoptosis and Wnt/β-catenin signaling pathway of SKOV3 human ovarian cancer cells. Methods The SKOV3 cells were treated with different concentrations of Sch B （ 0, 1.0, 10.0, 20.0, 50.0μmol/L） . The MTT was used to measure the proliferation inhibition rates at 24, 48, 72 and 96h treated with different concentrations of Sch B. The Annexin-FITC/PI double staining was employed to detect cell apoptosis at 48h after treat-ment with different concentrations of Sch B. The cycle distribution at 48h after treatment with Sch B was detected by flow cytometry. The Western blotting was used to measure the nuclearβ-catenin protein levels in Wnt/β-catenin signaling pathway as well as its downstream target C-myc and cyclin D1. The cytoplasm/nucleus activities of glycogen synthase kinase 3β （ GSK-3β） at 48h after treatment with different concentrations of Sch B were measured by activity assay kit. Results The Sch B can increase the proliferation inhibition rates in a dose-and time-dependent manner, and elevate the early and late apoptosis and cytoplasm/nucleus activities of GSK-3βat 48h after treatment. In addition to 1.0μmol/L, the proportion of cells in G0/G1 phase of the remaining concentrations were higher than those of 0μmo/L （ P〈0.05） , and the proportion of cells in S phase and G2/M phase andβ-catenin, C-myc and Cyclin D1 protein levels were lower than those of 0μmo/L （ P〈0.05） . Conclusion Sch B can inhibit the proliferation, promote apoptosis and cell cycle arrest and inhibit the activation of Wnt/β-catenin pathway.

关 键 词：SCHISANDRIN B 卵巢癌 增殖 凋亡 WNT Β-CATENIN信号通路 

分 类 号：R737.31[医药卫生—肿瘤]