白胡椒挥发油的超临界CO_2萃取及其GC-MS指纹图谱的建立  

Research on Supercritical CO_2 Fluid Extraction of Piper nigrum L. and Its GC-MS Fingerprints

作　　者：肜霖[1,2] 张敦铁[2] 夏秋冬[1,2] 朱巍[1,2] 赵同林[1,2] 吴昭[2] 谭烨[2] 邵兴伟[2] 黄龙[1,2] 

机构地区：[1]武汉黄鹤楼香精香料有限公司,湖北武汉430040 [2]湖北中烟工业有限责任公司,湖北武汉430040

出　　处：《香料香精化妆品》2014年第4期1-6,共6页Flavour Fragrance Cosmetics

摘　　要：为开发新的天然烟用香料,研究了超临界CO2萃取白胡椒挥发油的工艺,采用GC-MS法分析了白胡椒挥发油的成分,同时建立了挥发油GC-MS指纹图谱,并进行了方法学考察。结果表明:1超临界CO2萃取白胡椒挥发油的最佳工艺条件为萃取温度45℃、萃取压力35 MPa、萃取时间2 h,原料粉碎至粒径0.3 mm;2以GC-MS方法分析白胡椒挥发油共鉴定出66种成分,主要是萜烯类、醇类、酸类物质;312个主要成分的出峰次序及其相对含量构成了超临界CO2萃取白胡椒挥发油的特征指纹图谱;4利用所建立的指纹图谱,可以控制超临界CO2萃取白胡椒挥发油的质量及稳定性。In order to develop new natural cigarette flavors, Piper nigrum L. was extracted with supercriticaI CO2. The obtained oil was analyzed by GC-MS,and its fingerprints was estabilished, the method was investigated by methodological approach as well . The results showed that： ①the optimum extraction conditions were extraction temperature 45 C, extraction pressure 35 MPa,extraction time 2 h, the appropriate particle size 0.3 mm; ②66 components in the extract were identified by GC-MS, mainly including terpenes, alcohols, acids and esters; ③12 common peaks with their retention time and peak area of the components appeared the chromatographic fingerprints of this oil; ④ the fingerprints would be beneficial for the quality and stability control for piper oil obtained by suppercritical CO2 fluid extraction method.

关 键 词：白胡椒 超临界CO2萃取 气相色谱-质谱联用 指纹图谱 

分 类 号：R284.1[医药卫生—中药学] O658.2[医药卫生—中医学]