剑麻残渣皂苷元高产率菌株的筛选及特性研究  

Study on Screening and Speciality of Microorganism Degradating Sisalagenin to Produce Tigogenin from Sisal Residue

作　　者：孙昊[1] 刘建国[1] 朱桂梅[1] 李海云[1] 郝再彬[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,中国桂林541004

出　　处：《湖南师范大学自然科学学报》2014年第4期29-32,共4页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(31060193);国家麻类产业技术体系建设专项基金资助项目(CARS-19)

摘　　要：通过微生物发酵法将剑麻皂苷转化成皂苷元.采集桂林喀斯特地貌土壤样品,从能够降解皂苷糖基的多个菌株中筛选出JM-7,对其发酵条件进行优化,得到最适条件:温度30℃、pH为6、发酵时间5 d、底物质量浓度6 g/L,并且对产物皂苷元进行了HPLC检测和单晶衍射测定.Microbial fermentation method was used for producing tigogenin , the microorganism JM-7 was ob-tained from soil sample collected form Guangxi province via multiple screening .The fermentation conditions were optimized , and the results showed the optimum temperature was 30 ℃, the optimum pH was 6 , the best fermenta-tion time was 5 days, and the optimum substrate concentration was 6 g/L.HPLC and single crystal diffraction method were used to detect the fermented product .

关 键 词：剑麻皂苷 皂苷元 菌株筛选 发酵优化 

分 类 号：Q93[生物学—微生物学] S567[农业科学—中草药栽培]