核受体LXRα在HBV阳性肝硬化及原发性肝癌组织中的表达及意义  

Expression and clinical significance of LXRα in HBV infection-associated liver cirrhosis and hepatocellular carcinoma

作　　者：郑燕珊[1] 倪仰鹏[2] 陈理明[3] 陆军[4] 程訸 徐涵[1] 牛永东[1,5] 

机构地区：[1]汕头大学医学院药理教研室,广东汕头515041 [2]揭阳市人民医院病理科,广东汕头522000 [3]汕头大学医学院第一附属医院肿瘤科,广东汕头515041 [4]汕头大学医学院第一附属医院肝胆外科,广东汕头515041 [5]上海市肿瘤研究所"癌基因及相关基因国家重点实验室",上海200032

出　　处：《海南医学》2014年第15期2185-2188,共4页Hainan Medical Journal

基　　金：中国博士后科学基金(编号:2012M510200);广东省自然科学基金(编号:S2012010010955);上海市肿瘤研究所"癌基因及相关基因国家重点实验室"开放课题(编号:90-14-03);中央财政支持地方高校发展专项资金

摘　　要：目的检测肝脏X受体(Liver X receptor alpha,LXRα)在HBV阳性肝硬化组织及原发性肝癌中的表达情况及临床意义。方法收集20例揭阳市人民医院及汕头大学医学院第一附属医院手术切除的HBV阳性肝硬化原发性肝癌组织标本,应用免疫组化染色检测LXRα表达。Huh7细胞中分别过表达小鼠mLXRα,及共过表达HBx(HBV X protein)和mLXRα,并检测mLXRα及HBx对内源性LXRα信号通路下游靶基因SREBP-1(Sterol regulatory element-binding protein 1)的影响;双荧光素酶报告系统检测人肝癌Huh7细胞中HBx对LXRα转录活性的影响。结果 LXRα在肝硬化组织中阳性表达率为95%,高于癌组织的25%。HBx上调SREBP-1的表达可能与HBx调控LXRα的转录活性有关。结论 HBx通过调节癌前高表达的LXRα的转录活性,并影响其信号通路可能与HCC发生相关。Objective To detect the expression of liver X receptor alpha （LXRα） in HBV infection associated liver cirrhosis and hepatocellular carcinoma tissues and explore its clinical significance. Methods The expression of LXRαin 20 cases of HBV infection-associated liver cirrhosis and hepatocellular carcinoma specimens from Jiey-ang People＇s Hospital and the First Affiliated Hospital of Medical College, Shantou University was detected by immu-nohistochemical staining. Endogenous SREBP-1, a typical LXRα target gene, was detected by real-time PCR after over-expressing mice mLXRα, HBx and mLXRαin Huh7 cells. The transcriptional regulation of LXRαsignaling by HBx was evaluated by dual luciferase reporter system. Results The positive expression rate of LXRαwas 95%in cirrhosis, which was higher than that in HCC tissue with only 25%. The up-regulation of SREBP-1 expression by HBx may be related with the transcriptional activation of LXRαby HBx. Conclusion HBx maybe associated with the development of HCC by regulation of the transcriptional activation of LXRa, which was higher in cirrhosis, and ac-tivating the signal pathway.

关 键 词：LXRΑ HBX 原发性肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]