Bioanalysis and Pharmacokinetics of Eight Active Components from Huanglian Jiedu Decoction in Rat Plasma by LC-ESI-MS/MS Method  

作　　者：Cheng-cheng Peng Shu-ping Wang Hui-zi Jin Jian-fei Tao Guo-wei Wang Pan-lei Wei Xu-feng Zhang Le Li Run-hui Liu Wei-dong Zhang 

机构地区：[1]School of Pharmacy,Shanghai Jiao Tong University [2]School of Pharmacy,Second Military Medical University [3]Pharmacy Department,Shanghai Yangsi Hosipital [4]School of Chemistry and Environmental Engineering,Shanghai Institute of Technology

出　　处：《Chinese Herbal Medicines》2014年第3期198-210,共13页中草药（英文版）

基　　金：Program NCET Foundation NSFC(81230090);Global Research Network for Medicinal Plants and King Saud University,Shanghai Leading Academic Discipline Project(B906);Key laboratory of drug research for special environments,PLA,Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products(10DZ2251300);Scientific Foundation of Shanghai China(12401900801,09DZ1975700,09DZ1971500,10DZ1971700);National Major Project of China(2011ZX09307-002-03);National Key Technology R&D Program of China(2012BAI29B06)

摘　　要：Objective To develop a sensitive and rapid liquid chromatography-electrospray ionization-tandem mass spectrometry （LC-ESI-MS/MS） method for the simultaneous determination of wogonin, coptisine, berberine, palmatine, jatrorrhizine, phellodendrine, magnoflorine, and wogonoside in rat plasma and to evaluate the pharmacokinetic characteristics of Huanglian Jiedu Decoction （HJD）. Methods LC separation was performed on an Acquity HSS T3 column （100 mm × 2.1 mm, 1.8 μm） using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The detection was accomplished by using positive electrospray ionization in multiple-reaction monitoring mode. Plasma samples were pretreated by protein precipitation. Results The method showed a good linearity over a wide concentration range （r^2 〉 0.99）. The lower limits of quantification were 0.20 ng/mL for coptisine and phellodendrine, 0.48 ng/mL for berberine, 0.10 ng/mL for jatrorrhizine, 0.32 ng/mL for magnoflorine, 0.30 ng/mL for palmatine, and 4.80 ng/mL for wogonin and wogonoside, respectively. The intra- and inter-day precision of the analytes was less than 12.11%, while the accuracy was between -14.46% and 4.86%. The mean recovery of all the analytes ranged from 93.10% to 110.91%. Conclusion This validated method offers thee advantages of high sensitivity. It is successfully applied to evaluating the pharmacokinetic properties of HJD.Objective To develop a sensitive and rapid liquid chromatography-electrospray ionization-tandem mass spectrometry （LC-ESI-MS/MS） method for the simultaneous determination of wogonin, coptisine, berberine, palmatine, jatrorrhizine, phellodendrine, magnoflorine, and wogonoside in rat plasma and to evaluate the pharmacokinetic characteristics of Huanglian Jiedu Decoction （HJD）. Methods LC separation was performed on an Acquity HSS T3 column （100 mm × 2.1 mm, 1.8 μm） using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The detection was accomplished by using positive electrospray ionization in multiple-reaction monitoring mode. Plasma samples were pretreated by protein precipitation. Results The method showed a good linearity over a wide concentration range （r^2 〉 0.99）. The lower limits of quantification were 0.20 ng/mL for coptisine and phellodendrine, 0.48 ng/mL for berberine, 0.10 ng/mL for jatrorrhizine, 0.32 ng/mL for magnoflorine, 0.30 ng/mL for palmatine, and 4.80 ng/mL for wogonin and wogonoside, respectively. The intra- and inter-day precision of the analytes was less than 12.11%, while the accuracy was between -14.46% and 4.86%. The mean recovery of all the analytes ranged from 93.10% to 110.91%. Conclusion This validated method offers thee advantages of high sensitivity. It is successfully applied to evaluating the pharmacokinetic properties of HJD.

关 键 词：ALKALOID BIOANALYSIS flavonoid Huanglian Jiedu Decoction LC-ESI-MS/MS PHARMACOKINETICS 

分 类 号：R285.5[医药卫生—中药学]